Targeted Next Generation Sequencing for Human Papillomavirus Genotyping in Cervical Liquid-Based Cytology Samples.
HPV genotyping
atypical squamous cells of undetermined significance
cervical cancer screening
human papillomavirus
next generation sequencing
Journal
Cancers
ISSN: 2072-6694
Titre abrégé: Cancers (Basel)
Pays: Switzerland
ID NLM: 101526829
Informations de publication
Date de publication:
27 Jan 2022
27 Jan 2022
Historique:
received:
30
12
2021
revised:
21
01
2022
accepted:
26
01
2022
entrez:
15
2
2022
pubmed:
16
2
2022
medline:
16
2
2022
Statut:
epublish
Résumé
At present, human papillomavirus (HPV) testing is replacing morphology-based cytology as the primary tool for cervical cancer screening in several countries. However, the HPV assays approved for screening lack detection for all but one of the possibly carcinogenic HPV types and do not genotype all included HPV types. This study demonstrates the use of a targeted HPV next generation sequencing (NGS) panel to detect and genotype all 25 carcinogenic, probably carcinogenic, and possibly carcinogenic HPV types as well as the low-risk types HPV6 and HPV11. The panel was validated using a cohort of 93 paired liquid-based cytology samples (general practitioner (GP)-collected cervical samples and cervico-vaginal self-samples (SS)). Overall, the targeted panel had a sensitivity (GP = 97.7%, SS = 92.1%) and specificity (GP = 98.0%, SS = 96.4%) similar to the commercial HPV assays, Cobas
Identifiants
pubmed: 35158920
pii: cancers14030652
doi: 10.3390/cancers14030652
pmc: PMC8833452
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Vilhelm Pedersen og Hustrus Mindelegat
ID : 0065273
Organisme : Harboefonden
ID : 20195
Organisme : Fabrikant Einar Willumsens Mindelegat
ID : 500028
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