Development of Transient Recombinant Expression and Affinity Chromatography Systems for Human Fibrinogen.
affinity chromatography
fibrin
fibrinogen
hemostasis
recombinant protein
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
19 Jan 2022
19 Jan 2022
Historique:
received:
22
12
2021
revised:
11
01
2022
accepted:
14
01
2022
entrez:
15
2
2022
pubmed:
16
2
2022
medline:
9
4
2022
Statut:
epublish
Résumé
Fibrin forms the structural scaffold of blood clots and has great potential for biomaterial applications. Creating recombinant expression systems of fibrinogen, fibrin's soluble precursor, would advance the ability to construct mutational libraries that would enable structure-function studies of fibrinogen and expand the utility of fibrin as a biomaterial. Despite these needs, recombinant fibrinogen expression systems, thus far, have relied on the time-consuming creation of stable cell lines. Here we present tests of a transient fibrinogen expression system that can rapidly generate yields of 8-12 mg/L using suspension HEK Expi293
Identifiants
pubmed: 35162976
pii: ijms23031054
doi: 10.3390/ijms23031054
pmc: PMC8835685
pii:
doi:
Substances chimiques
Biocompatible Materials
0
Fibrin
9001-31-4
Fibrinogen
9001-32-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NHLBI NIH HHS
ID : R15 HL148842
Pays : United States
Organisme : NHLBI NIH HHS
ID : R15 HL150666
Pays : United States
Organisme : National Heart Lung and Blood Institute
ID : HL148842
Organisme : National Heart Lung and Blood Institute
ID : HL150666
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