Native Liquid Chromatography and Mass Spectrometry to Structurally and Functionally Characterize Endo-Xylanase Proteoforms.
Aspergillus niger
/ genetics
Chromatography, Gel
/ methods
Chromatography, Ion Exchange
/ methods
Chromatography, Liquid
/ methods
Endo-1,4-beta Xylanases
/ metabolism
Glycosylation
Mass Spectrometry
/ methods
Models, Molecular
Protein Processing, Post-Translational
Proteins
/ metabolism
Structure-Activity Relationship
boronate affinity chromatography
endo-1,4-β-xylanase
glycation
ion exchange chromatography
mass spectrometry
native protein analysis
size exclusion chromatography
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
24 Jan 2022
24 Jan 2022
Historique:
received:
24
12
2021
revised:
17
01
2022
accepted:
21
01
2022
entrez:
15
2
2022
pubmed:
16
2
2022
medline:
8
3
2022
Statut:
epublish
Résumé
Xylanases are of great value in various industries, including paper, food, and biorefinery. Due to their biotechnological production, these enzymes can contain a variety of post-translational modifications, which may have a profound effect on protein function. Understanding the structure-function relationship can guide the development of products with optimal performance. We have developed a workflow for the structural and functional characterization of an endo-1,4-β-xylanase (ENDO-I) produced by
Identifiants
pubmed: 35163230
pii: ijms23031307
doi: 10.3390/ijms23031307
pmc: PMC8835838
pii:
doi:
Substances chimiques
Proteins
0
Endo-1,4-beta Xylanases
EC 3.2.1.8
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Dutch Research Council
ID : 731.017.202
Pays : Netherlands
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