Synthetic virions reveal fatty acid-coupled adaptive immunogenicity of SARS-CoV-2 spike glycoprotein.

A549 Cells Allosteric Site / genetics Amino Acid Sequence Antibodies, Neutralizing / immunology Antibodies, Viral / immunology Binding Sites / genetics COVID-19 / immunology Cells, Cultured Cryoelectron Microscopy / methods Electron Microscope Tomography / methods Fatty Acid-Binding Proteins / immunology Fatty Acids / immunology Humans MCF-7 Cells Microscopy, Confocal / methods Protein Binding SARS-CoV-2 / immunology Sequence Homology, Amino Acid Spike Glycoprotein, Coronavirus / genetics Virion / immunology

Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
14 02 2022
Historique:
received: 09 07 2021
accepted: 24 01 2022
entrez: 15 2 2022
pubmed: 16 2 2022
medline: 1 3 2022
Statut: epublish

Résumé

SARS-CoV-2 infection is a major global public health concern with incompletely understood pathogenesis. The SARS-CoV-2 spike (S) glycoprotein comprises a highly conserved free fatty acid binding pocket (FABP) with unknown function and evolutionary selection advantage

Identifiants

DOI: 10.1038/s41467-022-28446-x PMID: 35165285 PMC: PMC8844029
pubmed: 35165285
doi: 10.1038/s41467-022-28446-x
pii: 10.1038/s41467-022-28446-x
pmc: PMC8844029
doi:

Substances chimiques

Antibodies, Neutralizing 0
Antibodies, Viral 0
Fatty Acid-Binding Proteins 0
Fatty Acids 0
Spike Glycoprotein, Coronavirus 0
spike glycoprotein, SARS-CoV 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

868

Subventions

Organisme : Bundesministerium für Bildung und Forschung (Federal Ministry of Education and Research)
ID : MaxSynBio Consortium
Organisme : Deutsche Forschungsgemeinschaft (German Research Foundation)
ID : SFB 1129
Organisme : Deutsche Forschungsgemeinschaft (German Research Foundation)
ID : Gottfried-Wilhelm-Leibniz Program
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Max-Planck-Gesellschaft (Max Planck Society)
ID : Max Planck School Matter to Life
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/L01386X/1
Pays : United Kingdom

Informations de copyright

© 2022. The Author(s).

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Auteurs

Oskar Staufer (O)

Department for Cellular Biophysics, Max Planck Institute for Medical Research, Jahnstraße 29, 69120, Heidelberg, Germany. oskar.staufer@mr.mpg.de.
Institute for Molecular Systems Engineering, University of Heidelberg, Im Neuenheimer Feld 253, 69120, Heidelberg, Germany. oskar.staufer@mr.mpg.de.
Max Planck-Bristol Center for Minimal Biology, University of Bristol, 1 Tankard's Close, Bristol, BS8 1TD, UK. oskar.staufer@mr.mpg.de.
Max Planck School Matter to Life, Jahnstraße 29, 69120, Heidelberg, Germany. oskar.staufer@mr.mpg.de.
ORCID: 0000-0002-8015-3132

Kapil Gupta (K)

School of Biochemistry, Biomedical Sciences, University of Bristol, 1 Tankard's Close, Bristol, BS8 1TD, UK.
Bristol Synthetic Biology Centre BrisSynBio, University of Bristol, 4 Tyndall Ave, Bristol, BS8 1TQ, UK.
ORCID: 0000-0002-2115-3503

Jochen Estebano Hernandez Bücher (JE)

Department for Cellular Biophysics, Max Planck Institute for Medical Research, Jahnstraße 29, 69120, Heidelberg, Germany.
Institute for Molecular Systems Engineering, University of Heidelberg, Im Neuenheimer Feld 253, 69120, Heidelberg, Germany.

Fabian Kohler (F)

Department of Physics, Technical University of Munich, 85748, Garching, Germany.

Christian Sigl (C)

Department of Physics, Technical University of Munich, 85748, Garching, Germany.

Gunjita Singh (G)

School of Biochemistry, Biomedical Sciences, University of Bristol, 1 Tankard's Close, Bristol, BS8 1TD, UK.

Kate Vasileiou (K)

School of Biochemistry, Biomedical Sciences, University of Bristol, 1 Tankard's Close, Bristol, BS8 1TD, UK.

Ana Yagüe Relimpio (A)

Department for Cellular Biophysics, Max Planck Institute for Medical Research, Jahnstraße 29, 69120, Heidelberg, Germany.
Institute for Molecular Systems Engineering, University of Heidelberg, Im Neuenheimer Feld 253, 69120, Heidelberg, Germany.

Meline Macher (M)

Department for Cellular Biophysics, Max Planck Institute for Medical Research, Jahnstraße 29, 69120, Heidelberg, Germany.
Institute for Molecular Systems Engineering, University of Heidelberg, Im Neuenheimer Feld 253, 69120, Heidelberg, Germany.
Max Planck School Matter to Life, Jahnstraße 29, 69120, Heidelberg, Germany.
ORCID: 0000-0002-9184-4959

Sebastian Fabritz (S)

Department for Chemical Biology, Max Planck Institute for Medical Research, Jahnstraße 29, 69120, Heidelberg, Germany.

Hendrik Dietz (H)

Max Planck School Matter to Life, Jahnstraße 29, 69120, Heidelberg, Germany.
Department of Physics, Technical University of Munich, 85748, Garching, Germany.
ORCID: 0000-0003-1270-3662

Elisabetta Ada Cavalcanti Adam (EA)

Department for Cellular Biophysics, Max Planck Institute for Medical Research, Jahnstraße 29, 69120, Heidelberg, Germany.
Max Planck School Matter to Life, Jahnstraße 29, 69120, Heidelberg, Germany.
ORCID: 0000-0003-0243-1552

Christiane Schaffitzel (C)

School of Biochemistry, Biomedical Sciences, University of Bristol, 1 Tankard's Close, Bristol, BS8 1TD, UK.
Bristol Synthetic Biology Centre BrisSynBio, University of Bristol, 4 Tyndall Ave, Bristol, BS8 1TQ, UK.
Halo Therapeutics Ltd, Science Creates, Albert Road St. Philips Central, Bristol, BS2 0XJ, UK.
ORCID: 0000-0002-1516-9760

Alessia Ruggieri (A)

Department of Infectious Diseases, Molecular Virology, Center for Integrated Infectious Disease Research, University of Heidelberg, Im Neuenheimer Feld 344, 69120, Heidelberg, Germany.
ORCID: 0000-0001-9981-3308

Ilia Platzman (I)

Department for Cellular Biophysics, Max Planck Institute for Medical Research, Jahnstraße 29, 69120, Heidelberg, Germany.
Institute for Molecular Systems Engineering, University of Heidelberg, Im Neuenheimer Feld 253, 69120, Heidelberg, Germany.
Max Planck-Bristol Center for Minimal Biology, University of Bristol, 1 Tankard's Close, Bristol, BS8 1TD, UK.

Imre Berger (I)

Max Planck-Bristol Center for Minimal Biology, University of Bristol, 1 Tankard's Close, Bristol, BS8 1TD, UK. imre.berger@bristol.ac.uk.
School of Biochemistry, Biomedical Sciences, University of Bristol, 1 Tankard's Close, Bristol, BS8 1TD, UK. imre.berger@bristol.ac.uk.
Bristol Synthetic Biology Centre BrisSynBio, University of Bristol, 4 Tyndall Ave, Bristol, BS8 1TQ, UK. imre.berger@bristol.ac.uk.
Halo Therapeutics Ltd, Science Creates, Albert Road St. Philips Central, Bristol, BS2 0XJ, UK. imre.berger@bristol.ac.uk.
ORCID: 0000-0001-7518-9045

Joachim P Spatz (JP)

Department for Cellular Biophysics, Max Planck Institute for Medical Research, Jahnstraße 29, 69120, Heidelberg, Germany. spatz@mr.mpg.de.
Institute for Molecular Systems Engineering, University of Heidelberg, Im Neuenheimer Feld 253, 69120, Heidelberg, Germany. spatz@mr.mpg.de.
Max Planck-Bristol Center for Minimal Biology, University of Bristol, 1 Tankard's Close, Bristol, BS8 1TD, UK. spatz@mr.mpg.de.
Max Planck School Matter to Life, Jahnstraße 29, 69120, Heidelberg, Germany. spatz@mr.mpg.de.
ORCID: 0000-0003-3419-9807

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Classifications MeSH

questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules A549 › Synthetic virions reveal fatty acid-coupled...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Sites de fixation › Site allostérique › Synthetic virions reveal fatty acid-coupled...
questionsmedicales.fr › Sciences de l'information › Sources d'information › Services d'information › Documentation › Données de séquences moléculaires › Séquence d'acides aminés › Synthetic virions reveal fatty acid-coupled...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Anticorps neutralisants › Synthetic virions reveal fatty acid-coupled...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Anticorps antiviraux › Synthetic virions reveal fatty acid-coupled...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Sites de fixation › Synthetic virions reveal fatty acid-coupled...
questionsmedicales.fr › Maladies de l'appareil respiratoire › Infections de l'appareil respiratoire › Pneumopathie infectieuse › Pneumopathie virale › COVID-19 › Synthetic virions reveal fatty acid-coupled...
questionsmedicales.fr › Cellules › Cellules cultivées › Synthetic virions reveal fatty acid-coupled...
questionsmedicales.fr › Techniques d'investigation › Microscopie électronique › Cryomicroscopie électronique › Synthetic virions reveal fatty acid-coupled...
questionsmedicales.fr › Techniques d'investigation › Microscopie électronique › Microscopie électronique à transmission › Tomographie en microscopie électronique › Synthetic virions reveal fatty acid-coupled...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de transport › Protéines de liaison aux acides gras › Synthetic virions reveal fatty acid-coupled...
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