Activated platelets and platelet-leukocyte aggregates in the equine systemic inflammatory response syndrome.
CD62P
P-selectin
equine systemic inflammatory response syndrome
flow cytometry
horses
platelets
Journal
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
ISSN: 1943-4936
Titre abrégé: J Vet Diagn Invest
Pays: United States
ID NLM: 9011490
Informations de publication
Date de publication:
May 2022
May 2022
Historique:
pubmed:
17
2
2022
medline:
4
5
2022
entrez:
16
2
2022
Statut:
ppublish
Résumé
In humans, activated platelets contribute to sepsis complications and to multiple organ failure. In our prospective analytical study of cases of the equine systemic inflammatory response syndrome (SIRS), we adapted a standard human protocol for the measurement of activated platelets and platelet-leukocyte aggregates (PLAs) in equine platelet-leukocyte-rich plasma (PLRP) by flow cytometry, and we investigated the hypothesis that activated platelets and PLAs are increased in clinical cases of SIRS. We included 17 adult horses and ponies fulfilling at least 2 SIRS criteria, and 10 healthy equids as controls. Activation of platelets was determined by increased expression of CD62P on platelets. Activated platelets and PLAs were measured before and after in vitro activation of platelets with collagen. Median expression of CD62P on platelets was significantly increased after activation in the control group: 1.45% (interquartile range [IQR]: 1.08-1.99%) initially versus 8.78% (IQR: 6.79-14.78%,
Identifiants
pubmed: 35168432
doi: 10.1177/10406387221077969
pmc: PMC9066687
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
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