Interactions among 17 respiratory pathogens: a cross-sectional study using clinical and community surveillance data.


Journal

medRxiv : the preprint server for health sciences
Titre abrégé: medRxiv
Pays: United States
ID NLM: 101767986

Informations de publication

Date de publication:
06 Feb 2022
Historique:
pubmed: 17 2 2022
medline: 17 2 2022
entrez: 16 2 2022
Statut: epublish

Résumé

Co-circulating respiratory pathogens can interfere with or promote each other, leading to important effects on disease epidemiology. Estimating the magnitude of pathogen-pathogen interactions from clinical specimens is challenging because sampling from symptomatic individuals can create biased estimates. We conducted an observational, cross-sectional study using samples collected by the Seattle Flu Study between 11 November 2018 and 20 August 2021. Samples that tested positive via RT-qPCR for at least one of 17 potential respiratory pathogens were included in this study. Semi-quantitative cycle threshold (Ct) values were used to measure pathogen load. Differences in pathogen load between monoinfected and coinfected samples were assessed using linear regression adjusting for age, season, and recruitment channel. 21,686 samples were positive for at least one potential pathogen. Most prevalent were rhinovirus (33·5%), Viral load data can be used to overcome sampling bias in studies of pathogen-pathogen interactions. When applied to respiratory pathogens, we found evidence of viral-

Sections du résumé

Background UNASSIGNED
Co-circulating respiratory pathogens can interfere with or promote each other, leading to important effects on disease epidemiology. Estimating the magnitude of pathogen-pathogen interactions from clinical specimens is challenging because sampling from symptomatic individuals can create biased estimates.
Methods UNASSIGNED
We conducted an observational, cross-sectional study using samples collected by the Seattle Flu Study between 11 November 2018 and 20 August 2021. Samples that tested positive via RT-qPCR for at least one of 17 potential respiratory pathogens were included in this study. Semi-quantitative cycle threshold (Ct) values were used to measure pathogen load. Differences in pathogen load between monoinfected and coinfected samples were assessed using linear regression adjusting for age, season, and recruitment channel.
Results UNASSIGNED
21,686 samples were positive for at least one potential pathogen. Most prevalent were rhinovirus (33·5%),
Conclusions UNASSIGNED
Viral load data can be used to overcome sampling bias in studies of pathogen-pathogen interactions. When applied to respiratory pathogens, we found evidence of viral-

Identifiants

pubmed: 35169816
doi: 10.1101/2022.02.04.22270474
pmc: PMC8845514
pii:
doi:

Types de publication

Preprint

Langues

eng

Subventions

Organisme : NIGMS NIH HHS
ID : R35 GM119774
Pays : United States

Auteurs

Roy Burstein (R)

Institute for Disease Modeling, Bill & Melinda Gates Foundation, Seattle WA USA.

Benjamin M Althouse (BM)

Institute for Disease Modeling, Bill & Melinda Gates Foundation, Seattle WA USA.
Institute for Disease Modeling, Bill & Melinda Gates Foundation, Seattle WA USA.
Department of Biology, New Mexico State University, Las Cruces, NM.

Amanda Adler (A)

Seattle Children's Research Institute, Seattle WA USA.

Adam Akullian (A)

Institute for Disease Modeling, Bill & Melinda Gates Foundation, Seattle WA USA.

Elizabeth Brandstetter (E)

Department of Medicine, University of Washington, Seattle WA USA.

Shari Cho (S)

Brotman Baty Institute for Precision Medicine, Seattle WA USA.

Anne Emanuels (A)

Department of Medicine, University of Washington, Seattle WA USA.

Kairsten Fay (K)

Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle WA USA.

Luis Gamboa (L)

Brotman Baty Institute for Precision Medicine, Seattle WA USA.

Peter Han (P)

Brotman Baty Institute for Precision Medicine, Seattle WA USA.

Kristen Huden (K)

Department of Medicine, University of Washington, Seattle WA USA.

Misja Ilcisin (M)

Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle WA USA.

Mandy Izzo (M)

Institute for Disease Modeling, Bill & Melinda Gates Foundation, Seattle WA USA.

Michael L Jackson (ML)

Kaiser Permanente Washington Health Research Institute, Seattle WA USA.

Ashley E Kim (AE)

Department of Medicine, University of Washington, Seattle WA USA.

Louise Kimball (L)

Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle WA USA.

Kirsten Lacombe (K)

Seattle Children's Research Institute, Seattle WA USA.

Jover Lee (J)

Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle WA USA.

Jennifer K Logue (JK)

Department of Medicine, University of Washington, Seattle WA USA.

Julia Rogers (J)

Department of Medicine, University of Washington, Seattle WA USA.

Erin Chung (E)

Department of Pediatrics, University of Washington, Seattle Children's Hospital, Seattle.

Thomas R Sibley (TR)

Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle WA USA.

Katrina Van Raay (K)

Brotman Baty Institute for Precision Medicine, Seattle WA USA.

Edward Wenger (E)

Institute for Disease Modeling, Bill & Melinda Gates Foundation, Seattle WA USA.

Caitlin R Wolf (CR)

Department of Medicine, University of Washington, Seattle WA USA.

Michael Boeckh (M)

Department of Medicine, University of Washington, Seattle WA USA.
Brotman Baty Institute for Precision Medicine, Seattle WA USA.
Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle WA USA.

Helen Chu (H)

Department of Medicine, University of Washington, Seattle WA USA.
Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle WA USA.

Jeff Duchin (J)

Department of Medicine, University of Washington, Seattle WA USA.
Public Health Seattle & King County, Seattle WA USA.

Mark Rieder (M)

Brotman Baty Institute for Precision Medicine, Seattle WA USA.

Jay Shendure (J)

Brotman Baty Institute for Precision Medicine, Seattle WA USA.
Department of Genome Sciences, University of Washington, Seattle WA USA.
Howard Hughes Medical Institute, Seattle WA USA.

Lea M Starita (LM)

Brotman Baty Institute for Precision Medicine, Seattle WA USA.
Department of Genome Sciences, University of Washington, Seattle WA USA.

Cecile Viboud (C)

Division of International Epidemiology and Population Studies, Fogarty International Center, National Institutes of Health, Bethesda, MD, USA.

Trevor Bedford (T)

Brotman Baty Institute for Precision Medicine, Seattle WA USA.
Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle WA USA.
Howard Hughes Medical Institute, Seattle WA USA.

Janet A Englund (JA)

Seattle Children's Research Institute, Seattle WA USA.
Brotman Baty Institute for Precision Medicine, Seattle WA USA.

Michael Famulare (M)

Institute for Disease Modeling, Bill & Melinda Gates Foundation, Seattle WA USA.

Classifications MeSH