Hydroxyl Radical Overproduction in the Envelope: an Achilles' Heel in Peptidoglycan Synthesis.
Fenton reaction
bacterial envelope biology
hydroxyl radical
iron homeostasis
oxidative stress
peptidoglycan
peptidoglycan synthesis
reactive oxygen species
Journal
Microbiology spectrum
ISSN: 2165-0497
Titre abrégé: Microbiol Spectr
Pays: United States
ID NLM: 101634614
Informations de publication
Date de publication:
23 02 2022
23 02 2022
Historique:
pubmed:
17
2
2022
medline:
4
3
2022
entrez:
16
2
2022
Statut:
ppublish
Résumé
While many mechanisms governing bacterial envelope homeostasis have been identified, others remain poorly understood. To decipher these processes, we previously developed an assay in the Gram-negative model Escherichia coli to identify genes involved in maintenance of envelope integrity. One such gene was ElyC, which was shown to be required for envelope integrity and peptidoglycan synthesis at room temperature. ElyC is predicted to be an integral inner membrane protein with a highly conserved domain of unknown function (DUF218). In this study, and stemming from a further characterization of the role of ElyC in maintaining cell envelope integrity, we serendipitously discovered an unappreciated form of oxidative stress in the bacterial envelope. We found that cells lacking ElyC overproduce hydroxyl radicals (HO
Identifiants
pubmed: 35170991
doi: 10.1128/spectrum.01203-21
pmc: PMC8849086
doi:
Substances chimiques
Escherichia coli Proteins
0
Peptidoglycan
0
Hydroxyl Radical
3352-57-6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0120321Références
J Org Chem. 2017 Mar 17;82(6):2817-2825
pubmed: 28248497
Genes Dev. 2018 May 1;32(9-10):602-619
pubmed: 29802123
Proc Natl Acad Sci U S A. 1989 May;86(10):3484-8
pubmed: 2471187
Science. 1988 Jun 3;240(4857):1302-9
pubmed: 3287616
J Biol Chem. 2003 Jan 31;278(5):3170-5
pubmed: 12419811
Mol Microbiol. 2019 Jul;112(1):233-248
pubmed: 31022322
J Bacteriol. 2007 Jun;189(12):4410-7
pubmed: 17449618
Redox Rep. 2001;6(4):229-34
pubmed: 11642713
Annu Rev Biochem. 2008;77:755-76
pubmed: 18173371
Annu Rev Microbiol. 2003;57:395-418
pubmed: 14527285
Crit Rev Anal Chem. 2016;46(2):160-9
pubmed: 26042844
Biol Chem. 2002 Mar-Apr;383(3-4):489-502
pubmed: 12033438
Environ Health Perspect. 1981 Aug;40:189-95
pubmed: 7274183
Nucleic Acids Res. 1991 Aug 25;19(16):4479-84
pubmed: 1653416
Annu Rev Physiol. 1986;48:657-67
pubmed: 3010829
Science. 1997 Sep 5;277(5331):1453-62
pubmed: 9278503
Biometals. 2008 Jun;21(3):321-32
pubmed: 17934697
J Biol Chem. 2013 Sep 13;288(37):26464-72
pubmed: 23861390
J Bacteriol. 2016 Dec 28;199(2):
pubmed: 27821607
Ann Clin Lab Sci. 1996 Mar-Apr;26(2):185-91
pubmed: 8852428
Mol Gen Genet. 1998 Jul;259(1):46-53
pubmed: 9738879
Proc Natl Acad Sci U S A. 2000 May 23;97(11):5978-83
pubmed: 10811905
Chem Phys Lipids. 2009 Jan;157(1):1-11
pubmed: 18977338
J Bacteriol. 2005 Aug;187(16):5723-31
pubmed: 16077119
Microbiol Mol Biol Rev. 1998 Mar;62(1):181-203
pubmed: 9529891
Dalton Trans. 2012 Mar 7;41(9):2820-34
pubmed: 22261733
Appl Environ Microbiol. 1995 Jun;61(6):2180-5
pubmed: 16535043
FEMS Microbiol Rev. 2014 Nov;38(6):1126-45
pubmed: 24923554
Microb Drug Resist. 2014 Jun;20(3):199-214
pubmed: 24799078
Nat Rev Microbiol. 2015 Jan;13(1):42-51
pubmed: 25435309
PLoS One. 2008 Feb 06;3(2):e1573
pubmed: 18253509
J Bacteriol. 1989 Jun;171(6):3008-15
pubmed: 2656637
Met Ions Biol Syst. 1999;36:521-45
pubmed: 10093935
Proc Natl Acad Sci U S A. 1998 Mar 3;95(5):2659-63
pubmed: 9482943
J Bacteriol. 1996 Jan;178(1):94-102
pubmed: 8550448
FEMS Microbiol Rev. 2016 Mar;40(2):273-98
pubmed: 26684538
J Bacteriol. 1991 May;173(9):2864-71
pubmed: 1708380
Science. 1988 Apr 29;240(4852):640-2
pubmed: 2834821
Science. 2018 Dec 7;362(6419):1186-1189
pubmed: 30361388
J Bacteriol. 2006 May;188(9):3371-81
pubmed: 16621832
Free Radic Biol Med. 2018 Nov 20;128:3-22
pubmed: 29567392
Mol Microbiol. 2018 Sep;109(6):826-844
pubmed: 29995990
IUBMB Life. 1999 Aug;48(2):157-61
pubmed: 10794591
J Bacteriol. 1989 Jul;171(7):3740-5
pubmed: 2500418
FEMS Microbiol Rev. 2014 Jul;38(4):633-59
pubmed: 24188101
Genes Dev. 1992 Dec;6(12B):2646-54
pubmed: 1340475
FEMS Microbiol Rev. 2001 May;25(3):335-47
pubmed: 11348688
FEMS Microbiol Rev. 2008 Mar;32(2):149-67
pubmed: 18194336
Environ Health Perspect. 1994 Sep;102 Suppl 3:231-6
pubmed: 7843104
J Biol Chem. 2002 Aug 2;277(31):27689-96
pubmed: 12016214
Nucleic Acids Res. 2008 Jan;36(Database issue):D281-8
pubmed: 18039703
Biochim Biophys Acta. 2010 Aug;1800(8):798-805
pubmed: 20138126
Int J Med Microbiol. 2010 Feb;300(2-3):161-9
pubmed: 20005776
FEMS Microbiol Rev. 2003 Jun;27(2-3):215-37
pubmed: 12829269
J Bacteriol. 1994 Sep;176(18):5631-8
pubmed: 8083157
PLoS Genet. 2014 Jan;10(1):e1004056
pubmed: 24391520
Prog Lipid Res. 2013 Jul;52(3):249-76
pubmed: 23500459
Toxicology. 2000 Aug 14;149(1):43-50
pubmed: 10963860
J Bacteriol. 2018 Sep 10;200(19):
pubmed: 30012727
J Bacteriol. 1992 Sep;174(17):5549-59
pubmed: 1512190
Chemotherapy. 2001 Jan-Feb;47(1):10-4
pubmed: 11125227
Methods Mol Biol. 2016;1440:11-27
pubmed: 27311661
J Biol Chem. 2018 Jun 1;293(22):8530-8542
pubmed: 29661935
Front Microbiol. 2021 Feb 15;12:627104
pubmed: 33658986
Microbiology (Reading). 1998 Dec;144 ( Pt 12):3297-3308
pubmed: 9884221
Proteins. 2008 Jul;72(1):506-9
pubmed: 18393394
Mol Microbiol. 2013 Jul;89(1):1-13
pubmed: 23679048
Proc Natl Acad Sci U S A. 1994 Dec 20;91(26):12438-42
pubmed: 7809055
Environ Mol Mutagen. 1991;18(1):22-7
pubmed: 1907540
Microbiol Rev. 1993 Sep;57(3):522-42
pubmed: 8246839
Elife. 2020 Jan 06;9:
pubmed: 31904338
Nat Rev Microbiol. 2013 Jul;11(7):443-54
pubmed: 23712352
EcoSal Plus. 2008 Sep;3(1):
pubmed: 26443738