Lipid and Nucleocapsid N-Protein Accumulation in COVID-19 Patient Lung and Infected Cells.
Adolescent
Aged
Animals
COVID-19
/ metabolism
Child, Preschool
Chlorocebus aethiops
Disease Outbreaks
Female
Fluorescent Antibody Technique
Humans
Immunohistochemistry
Lipid Metabolism
/ physiology
Lipids
/ analysis
Lung
/ cytology
Male
Microscopy, Immunoelectron
Middle Aged
Nucleocapsid
/ analysis
Rabbits
SARS-CoV-2
/ ultrastructure
Vero Cells
/ virology
COVID-19
electron microscopy
lipids
pathology
Journal
Microbiology spectrum
ISSN: 2165-0497
Titre abrégé: Microbiol Spectr
Pays: United States
ID NLM: 101634614
Informations de publication
Date de publication:
23 02 2022
23 02 2022
Historique:
pubmed:
17
2
2022
medline:
22
3
2022
entrez:
16
2
2022
Statut:
ppublish
Résumé
The pandemic of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global outbreak and prompted an enormous research effort. Still, the subcellular localization of the coronavirus in lungs of COVID-19 patients is not well understood. Here, the localization of the SARS-CoV-2 proteins is studied in postmortem lung material of COVID-19 patients and in SARS-CoV-2-infected Vero cells, processed identically. Correlative light and electron microscopy on semithick cryo-sections demonstrated induction of electron-lucent, lipid-filled compartments after SARS-CoV-2 infection in both lung and cell cultures. In lung tissue, the nonstructural protein 4 and the stable nucleocapsid N-protein were detected on these novel lipid-filled compartments. The induction of such lipid-filled compartments and the localization of the viral proteins in lung of patients with fatal COVID-19 may explain the extensive inflammatory response and provide a new hallmark for SARS-CoV-2 infection at the final, fatal stage of infection.
Identifiants
pubmed: 35171025
doi: 10.1128/spectrum.01271-21
pmc: PMC8849100
doi:
Substances chimiques
Lipids
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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