Intra-amniotic administration of l-glutamine promotes intestinal maturation and enteroendocrine stimulation in chick embryos.
Amniotic Fluid
Animal Nutritional Physiological Phenomena
/ physiology
Animals
Chick Embryo
/ cytology
Enteroendocrine Cells
/ drug effects
Glucagon-Like Peptide-2 Receptor
/ metabolism
Glutamine
/ administration & dosage
Injections
Insulin-Like Growth Factor I
/ metabolism
Intestinal Mucosa
/ embryology
Intestine, Small
/ embryology
Receptor, IGF Type 1
/ metabolism
Stimulation, Chemical
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
16 02 2022
16 02 2022
Historique:
received:
05
09
2021
accepted:
28
01
2022
entrez:
17
2
2022
pubmed:
18
2
2022
medline:
15
3
2022
Statut:
epublish
Résumé
Initial nutritional stimulation is a key driving force for small intestinal maturation. In chick embryos, administration of l-glutamine (Gln) into the amniotic fluid stimulates early development of the small intestinal epithelium by promoting enterocyte differentiation. In this study, we evaluated the effects of intra-amniotic administration of Gln on enterocyte morphology and function, and elucidated a potential enteroendocrine pathway through which Gln stimulates small intestinal maturation. Our results show that Gln stimulation at embryonic day 17 significantly increased enterocyte and microvilli dimensions by 10 and 20%, respectively, within 48 h. Post-hatch, enterocytes and microvilli were 20% longer in Gln-treated chicks. Correspondingly, Gln stimulation significantly upregulated mRNA expression of brush border nutrient transporters PepT-1 and SGLT-1 and tight junction proteins TJP-1 and TJP-2, before and after hatch (P < 0.05). Since GLP-2 signaling from intestinal L-cells is associated with enterocyte growth, functionality and integrity, we examined the effects of Gln stimulation on mRNA expression of key hormones and receptors within this enteroendocrine pathway and found significant increases in GLP-2R, IGF-1 and IGF-1R expression before and after hatch (P < 0.05). In conclusion, our findings link primary nutrient stimulation in the developing small intestine with enterocyte morphological and functional maturation and enteroendocrine signaling.
Identifiants
pubmed: 35173228
doi: 10.1038/s41598-022-06440-z
pii: 10.1038/s41598-022-06440-z
pmc: PMC8850624
doi:
Substances chimiques
Glucagon-Like Peptide-2 Receptor
0
Glutamine
0RH81L854J
Insulin-Like Growth Factor I
67763-96-6
Receptor, IGF Type 1
EC 2.7.10.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2645Informations de copyright
© 2022. The Author(s).
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