Surgical Procedure for Implantation of Human Tumor Tissue into the Epithelium-Free Mammary Fat Pad of Immunocompromised Mice to Generate Patient-Derived Xenografts (PDX).
Breast cancer
Cleared mammary fat pad
Epithelium-free mammary fat pad
Orthotopic transplantation
PDX
Patient-derived xenograft
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
17
2
2022
pubmed:
18
2
2022
medline:
22
2
2022
Statut:
ppublish
Résumé
Implantation of cancerous tissue obtained from patient biopsies or surgical procedures to create patient-derived xenograft models (PDX) has greatly enhanced the ability of scientists and physicians to perform translational research involving pre-clinical testing. PDX models are more representative of patient cancer tumors than traditional cell lines as they retain the three-dimensional architecture of the tumor, and are genomically, transcriptomically, and proteomically analogous. These models can be used for a variety of experiments to evaluate response of tumors to experimental therapeutic agents and to aid in the elucidation of response and resistance mechanisms of existing therapies. Orthotopic transplantation of breast cancer tissue into the mammary fat pad has been proven to be the most robust method of establishing breast cancer PDX models. Here we describe the procedure for implanting patient tumor tissue, or existing PDX tissue, into the mammary fat pad of immunocompromised mice, with notes, where necessary, for transplantation of normal or preneoplastic tissue.
Identifiants
pubmed: 35175598
doi: 10.1007/978-1-0716-2193-6_10
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
195-207Subventions
Organisme : NCI NIH HHS
ID : P30 CA125123
Pays : United States
Informations de copyright
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
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