Generation of human GAPDH knock-in reporter iPSC lines for stable expression of tdTomato in pluripotent and differentiated culture conditions.

CRISPR-Cas Systems / genetics Cell Differentiation Cell Line Humans Induced Pluripotent Stem Cells / metabolism Luminescent Proteins / metabolism Red Fluorescent Protein

Journal

Stem cell research
ISSN: 1876-7753
Titre abrégé: Stem Cell Res
Pays: England
ID NLM: 101316957

Informations de publication

Date de publication:
04 2022
Historique:
received: 12 12 2021
accepted: 03 02 2022
pubmed: 18 2 2022
medline: 16 4 2022
entrez: 17 2 2022
Statut: ppublish

Résumé

Human induced pluripotent stem cells (iPSCs) can differentiate into multiple cell types and are utilized for research on human development and regenerative medicine. Here, we report the establishment of human GAPDH knock-in reporter iPSC lines (GAPDH-tdT1 and 2), via CRISPR/Cas9-mediated homologous recombination, that stably express tdTomato as a constitutive cell label in both iPSCs and their differentiated derivatives. These cell lines will provide useful tools to trace cell locations and fates in 2D cultures and 3D organoids and will facilitate in vivo experiments.

Identifiants

DOI: 10.1016/j.scr.2022.102704 PMID: 35176664
pubmed: 35176664
pii: S1873-5061(22)00053-8
doi: 10.1016/j.scr.2022.102704
pii:
doi:

Substances chimiques

Luminescent Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

102704

Informations de copyright

Copyright © 2022 The Author(s). Published by Elsevier B.V. All rights reserved.

Auteurs

Daisuke Kamiya (D)

Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan; Takeda-CiRA Joint Program (T-CiRA), Fujisawa, Kanagawa, Japan. Electronic address: kamiya@cira.kyoto-u.ac.jp.

Teruyoshi Yamashita (T)

T-CiRA Discovery, Takeda Pharmaceutical Co. Ltd, Fujisawa, Kanagawa, Japan; Takeda-CiRA Joint Program (T-CiRA), Fujisawa, Kanagawa, Japan.

Teppei Akaboshi (T)

Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan; Takeda-CiRA Joint Program (T-CiRA), Fujisawa, Kanagawa, Japan.

Yoshiki Yamaguchi (Y)

Orizuru Therapeutics Inc, Fujisawa, Kanagawa, Japan.

Yayoi Toyooka (Y)

Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan; Takeda-CiRA Joint Program (T-CiRA), Fujisawa, Kanagawa, Japan.

Makoto Ikeya (M)

Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan; Takeda-CiRA Joint Program (T-CiRA), Fujisawa, Kanagawa, Japan.

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Systèmes CRISPR-Cas › Generation of human GAPDH knock-in reporter...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Différenciation cellulaire › Generation of human GAPDH knock-in reporter...
questionsmedicales.fr › Cellules › Cellules cultivées › Lignée cellulaire › Generation of human GAPDH knock-in reporter...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Generation of human GAPDH knock-in reporter...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches pluripotentes › Cellules souches pluripotentes induites › Generation of human GAPDH knock-in reporter...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines luminescentes › Generation of human GAPDH knock-in reporter...