Tracing transport of protein aggregates in microgravity versus unit gravity crystallization.
Journal
NPJ microgravity
ISSN: 2373-8065
Titre abrégé: NPJ Microgravity
Pays: United States
ID NLM: 101703605
Informations de publication
Date de publication:
17 Feb 2022
17 Feb 2022
Historique:
received:
20
01
2021
accepted:
20
12
2021
entrez:
18
2
2022
pubmed:
19
2
2022
medline:
19
2
2022
Statut:
epublish
Résumé
Microgravity conditions have been used to improve protein crystallization from the early 1980s using advanced crystallization apparatuses and methods. Early microgravity crystallization experiments confirmed that minimal convection and a sedimentation-free environment is beneficial for growth of crystals with higher internal order and in some cases, larger volume. It was however realized that crystal growth in microgravity requires additional time due to slower growth rates. The progress in space research via the International Space Station (ISS) provides a laboratory-like environment to perform convection-free crystallization experiments for an extended time. To obtain detailed insights in macromolecular transport phenomena under microgravity and the assumed reduction of unfavorable impurity incorporation in growing crystals, microgravity and unit gravity control experiments for three different proteins were designed. To determine the quantity of impurity incorporated into crystals, fluorescence-tagged aggregates of the proteins (acting as impurities) were prepared. The recorded fluorescence intensities of the respective crystals reveal reduction in the incorporation of aggregates under microgravity for different aggregate quantities. The experiments and data obtained, provide insights about macromolecular transport in relation to molecular weight of the target proteins, as well as information about associated diffusion behavior and crystal lattice formation. Results suggest one explanation why microgravity-grown protein crystals often exhibit higher quality. Furthermore, results from these experiments can be used to predict which proteins may benefit more from microgravity crystallization.
Identifiants
pubmed: 35177635
doi: 10.1038/s41526-022-00191-x
pii: 10.1038/s41526-022-00191-x
pmc: PMC8854672
doi:
Types de publication
Journal Article
Langues
eng
Pagination
4Subventions
Organisme : Intramural NASA
ID : 80NSSC17K0013
Pays : United States
Organisme : NASA | Glenn Research Center (NASA Glenn Research Center)
ID : 80NSSC18K0013
Informations de copyright
© 2022. The Author(s).
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