Cryo-EM structure of the nuclear ring from Xenopus laevis nuclear pore complex.

Animals Cryoelectron Microscopy Cytoplasm / metabolism Nuclear Envelope / metabolism Nuclear Pore / metabolism Nuclear Pore Complex Proteins / chemistry Xenopus Proteins / metabolism Xenopus laevis / metabolism

Journal

Cell research
ISSN: 1748-7838
Titre abrégé: Cell Res
Pays: England
ID NLM: 9425763

Informations de publication

Date de publication:
04 2022
Historique:
received: 25 11 2021
accepted: 21 12 2021
pubmed: 19 2 2022
medline: 6 4 2022
entrez: 18 2 2022
Statut: ppublish

Résumé

Nuclear pore complex (NPC) shuttles cargo across the nuclear envelope. Here we present single-particle cryo-EM structure of the nuclear ring (NR) subunit from Xenopus laevis NPC at an average resolution of 5.6 Å. The NR subunit comprises two 10-membered Y complexes, each with the nucleoporin ELYS closely associating with Nup160 and Nup37 of the long arm. Unlike the cytoplasmic ring (CR) or inner ring (IR), the NR subunit contains only one molecule each of Nup205 and Nup93. Nup205 binds both arms of the Y complexes and interacts with the stem of inner Y complex from the neighboring subunit. Nup93 connects the stems of inner and outer Y complexes within the same NR subunit, and places its N-terminal extended helix into the axial groove of Nup205 from the neighboring subunit. Together with other structural information, we have generated a composite atomic model of the central ring scaffold that includes the NR, IR, and CR. The IR is connected to the two outer rings mainly through Nup155. This model facilitates functional understanding of vertebrate NPC.

Identifiants

DOI: 10.1038/s41422-021-00610-w PMID: 35177819 PMC: PMC8976044
pubmed: 35177819
doi: 10.1038/s41422-021-00610-w
pii: 10.1038/s41422-021-00610-w
pmc: PMC8976044
doi:

Substances chimiques

NUP155 protein, Xenopus 0
Nuclear Pore Complex Proteins 0
Xenopus Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

349-358

Informations de copyright

© 2022. The Author(s).

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Auteurs

Gaoxingyu Huang (G)

Westlake Laboratory of Life Sciences and Biomedicine, 18 Shilongshan Road, Hangzhou, Zhejiang, China. huanggaoxingyu@westlake.edu.cn.
Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, 18 Shilongshan Road, Hangzhou, Zhejiang, China. huanggaoxingyu@westlake.edu.cn.
Institute of Biology, Westlake Institute for Advanced Study, 18 Shilongshan Road, Hangzhou, Zhejiang, China. huanggaoxingyu@westlake.edu.cn.

Xiechao Zhan (X)

Westlake Laboratory of Life Sciences and Biomedicine, 18 Shilongshan Road, Hangzhou, Zhejiang, China.
Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, 18 Shilongshan Road, Hangzhou, Zhejiang, China.
Institute of Biology, Westlake Institute for Advanced Study, 18 Shilongshan Road, Hangzhou, Zhejiang, China.

Chao Zeng (C)

Westlake Laboratory of Life Sciences and Biomedicine, 18 Shilongshan Road, Hangzhou, Zhejiang, China.
Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, 18 Shilongshan Road, Hangzhou, Zhejiang, China.
Institute of Biology, Westlake Institute for Advanced Study, 18 Shilongshan Road, Hangzhou, Zhejiang, China.

Xuechen Zhu (X)

Westlake Laboratory of Life Sciences and Biomedicine, 18 Shilongshan Road, Hangzhou, Zhejiang, China.
Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, 18 Shilongshan Road, Hangzhou, Zhejiang, China.
Institute of Biology, Westlake Institute for Advanced Study, 18 Shilongshan Road, Hangzhou, Zhejiang, China.

Ke Liang (K)

Westlake Laboratory of Life Sciences and Biomedicine, 18 Shilongshan Road, Hangzhou, Zhejiang, China.
Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, 18 Shilongshan Road, Hangzhou, Zhejiang, China.
Institute of Biology, Westlake Institute for Advanced Study, 18 Shilongshan Road, Hangzhou, Zhejiang, China.

Yanyu Zhao (Y)

Westlake Laboratory of Life Sciences and Biomedicine, 18 Shilongshan Road, Hangzhou, Zhejiang, China.
Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, 18 Shilongshan Road, Hangzhou, Zhejiang, China.
Institute of Biology, Westlake Institute for Advanced Study, 18 Shilongshan Road, Hangzhou, Zhejiang, China.

Pan Wang (P)

Beijing Advanced Innovation Center for Structural Biology & Frontier Research Center for Biological Structure, Tsinghua University, Beijing, China.
Tsinghua University-Peking University Joint Center for Life Sciences; School of Life Sciences, Tsinghua University, Beijing, China.

Qifan Wang (Q)

Westlake Laboratory of Life Sciences and Biomedicine, 18 Shilongshan Road, Hangzhou, Zhejiang, China.
Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, 18 Shilongshan Road, Hangzhou, Zhejiang, China.
Institute of Biology, Westlake Institute for Advanced Study, 18 Shilongshan Road, Hangzhou, Zhejiang, China.

Qiang Zhou (Q)

Westlake Laboratory of Life Sciences and Biomedicine, 18 Shilongshan Road, Hangzhou, Zhejiang, China.
Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, 18 Shilongshan Road, Hangzhou, Zhejiang, China.
Institute of Biology, Westlake Institute for Advanced Study, 18 Shilongshan Road, Hangzhou, Zhejiang, China.
ORCID: 0000-0002-6237-8813

Qinghua Tao (Q)

Beijing Advanced Innovation Center for Structural Biology & Frontier Research Center for Biological Structure, Tsinghua University, Beijing, China.

Minhao Liu (M)

Beijing Advanced Innovation Center for Structural Biology & Frontier Research Center for Biological Structure, Tsinghua University, Beijing, China.

Jianlin Lei (J)

Beijing Advanced Innovation Center for Structural Biology & Frontier Research Center for Biological Structure, Tsinghua University, Beijing, China.
ORCID: 0000-0002-9384-8742

Chuangye Yan (C)

Beijing Advanced Innovation Center for Structural Biology & Frontier Research Center for Biological Structure, Tsinghua University, Beijing, China.
Tsinghua University-Peking University Joint Center for Life Sciences; School of Life Sciences, Tsinghua University, Beijing, China.
ORCID: 0000-0001-9338-8048

Yigong Shi (Y)

Westlake Laboratory of Life Sciences and Biomedicine, 18 Shilongshan Road, Hangzhou, Zhejiang, China. syg@westlake.edu.cn.
Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, 18 Shilongshan Road, Hangzhou, Zhejiang, China. syg@westlake.edu.cn.
Institute of Biology, Westlake Institute for Advanced Study, 18 Shilongshan Road, Hangzhou, Zhejiang, China. syg@westlake.edu.cn.
Beijing Advanced Innovation Center for Structural Biology & Frontier Research Center for Biological Structure, Tsinghua University, Beijing, China. syg@westlake.edu.cn.
Tsinghua University-Peking University Joint Center for Life Sciences; School of Life Sciences, Tsinghua University, Beijing, China. syg@westlake.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Cryo-EM structure of the nuclear ring from...
questionsmedicales.fr › Techniques d'investigation › Microscopie électronique › Cryomicroscopie électronique › Cryo-EM structure of the nuclear ring from...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Cryo-EM structure of the nuclear ring from...
questionsmedicales.fr › Cellules › Structures cellulaires › Fractions subcellulaires › Membranes intracellulaires › Enveloppe nucléaire › Cryo-EM structure of the nuclear ring from...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Structures nucléaires › Pore nucléaire › Cryo-EM structure of the nuclear ring from...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Transporteurs nucléocytoplasmiques › Complexe protéique du pore nucléaire › Cryo-EM structure of the nuclear ring from...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines d'amphibien › Protéines de Xénope › Cryo-EM structure of the nuclear ring from...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Amphibiens › Anura › Pipidae › Xenopus › Xenopus laevis › Cryo-EM structure of the nuclear ring from...