Nanoencapsulated Lippia rotundifolia antimicrobial peptide: synthesis, characterization, antimicrobial activity, and cytotoxicity evaluations.
Antimicrobial peptides
Nanocapsulates
Nanotechnology
Journal
Archives of microbiology
ISSN: 1432-072X
Titre abrégé: Arch Microbiol
Pays: Germany
ID NLM: 0410427
Informations de publication
Date de publication:
18 Feb 2022
18 Feb 2022
Historique:
received:
16
08
2021
accepted:
31
01
2022
revised:
26
01
2022
entrez:
18
2
2022
pubmed:
19
2
2022
medline:
23
2
2022
Statut:
epublish
Résumé
Antimicrobial peptides (AMP) are promising novel antibiotics but exhibit low stability and can be toxic. The AMP encapsulation can be used to protect the drug and control its release rates. The Lr-AMP1f encapsulated into chitosan nanoparticle (NP) by ionic gelation method reached 90% efficiency. The results indicated that the hydrodynamic particle size of NPs increased from 196.1 ± 3.14 nm (free NP) to 228.1 ± 12.22 nm (nanoencapsulated Lr-AMP1f), while the atomic force microscope showed the spherical shape. The Zeta potential of the nanoencapsulated Lr-AMP1f was high (+ 35 mV). These AMP-loaded NPs exhibited stability for up to 21 days of storage. The minimum inhibitory concentration (MIC) of free Lr-AMP1f was 8 µg/mL for E. coli and S. epidermidis. However, the nanoencapsulated Lr-AMP1f produced a bacteriostatic effect against both bacteria at 8 µg/mL. The MIC of nanoencapsulated Lr-AMP1f was 16 µg/mL for E. coli and 32 for S. epidermidis. Nanoencapsulated Lr-AMP1f was nontoxic to HEK293 cells. Promisingly, chitosan NP can be used as a vehicle for the antibacterial application of new AMP (Lr-AMP1f).
Identifiants
pubmed: 35179654
doi: 10.1007/s00203-022-02787-z
pii: 10.1007/s00203-022-02787-z
doi:
Substances chimiques
Anti-Bacterial Agents
0
Antimicrobial Peptides
0
Chitosan
9012-76-4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
184Subventions
Organisme : Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
ID : Financial code: 001
Organisme : Fundação de Amparo à Pesquisa do Estado de Minas Gerais
ID : RED-00282-16
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 405030/2015-0
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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