Immunological characterization of the chemically prepared ghosts of Salmonella Typhimurium as a vaccine candidate.
Bacterial ghosts
Bacterial ghosts’ applications
Salmonella Typhimurium
Vaccination trial, immunological characterization
Journal
BMC veterinary research
ISSN: 1746-6148
Titre abrégé: BMC Vet Res
Pays: England
ID NLM: 101249759
Informations de publication
Date de publication:
18 Feb 2022
18 Feb 2022
Historique:
received:
29
06
2021
accepted:
13
12
2021
entrez:
19
2
2022
pubmed:
20
2
2022
medline:
7
4
2022
Statut:
epublish
Résumé
Bacterial ghosts are the evacuated bacterial cellular membranes from most of the genetic and protein contents which preserved their surface characters. Recently, bacterial ghosts exploited for different biomedical applications, for instance, vaccination. The purpose of this study is to measure the immunogenic protective response of bacterial ghosts of Salmonella Typhimurium in animals and to allow future testing this response in humans. The immunologic response was qualitatively, quantitatively, and functionally measured. We have measured the humoral and cellular immune responses, such as immunoglobulins elevation (IgG), increased granulocytes, serum antibacterial activity, clearance of virulence in feces and liver, and the survival rate. The bacterial ghosts' vaccine was able to protect 100% of subcutaneously vaccinated rats and 75% of adjuvant subcutaneously vaccinated rats. The lowest survival rate was in the orally vaccinated group (25%). The maximum level of serum IgG titers, as well as serum and feces bactericidal activity (100% eradication), was exhibited in the subcutaneously vaccinated group with adjuvant vaccines followed by the subcutaneously vaccinated one. Additionally, the highest granulocytes' number was observed in the adjuvant vaccine subcutaneously immunized group. The bacterial load in liver homogenate was eliminated in the subcutaneously vaccinated rats after the virulence challenge. The bacterial ghosts of Salmonella enterica serovar Typhimurium that prepared by Tween 80 Protocol showed an effective vaccine candidate that protected animals, eliminated the virulence in feces and liver. These findings show that chemically induced bacterial ghosts of Salmonella Typhimurium can be a promising vaccine.
Sections du résumé
BACKGROUND
BACKGROUND
Bacterial ghosts are the evacuated bacterial cellular membranes from most of the genetic and protein contents which preserved their surface characters. Recently, bacterial ghosts exploited for different biomedical applications, for instance, vaccination. The purpose of this study is to measure the immunogenic protective response of bacterial ghosts of Salmonella Typhimurium in animals and to allow future testing this response in humans. The immunologic response was qualitatively, quantitatively, and functionally measured. We have measured the humoral and cellular immune responses, such as immunoglobulins elevation (IgG), increased granulocytes, serum antibacterial activity, clearance of virulence in feces and liver, and the survival rate.
RESULTS
RESULTS
The bacterial ghosts' vaccine was able to protect 100% of subcutaneously vaccinated rats and 75% of adjuvant subcutaneously vaccinated rats. The lowest survival rate was in the orally vaccinated group (25%). The maximum level of serum IgG titers, as well as serum and feces bactericidal activity (100% eradication), was exhibited in the subcutaneously vaccinated group with adjuvant vaccines followed by the subcutaneously vaccinated one. Additionally, the highest granulocytes' number was observed in the adjuvant vaccine subcutaneously immunized group. The bacterial load in liver homogenate was eliminated in the subcutaneously vaccinated rats after the virulence challenge.
CONCLUSIONS
CONCLUSIONS
The bacterial ghosts of Salmonella enterica serovar Typhimurium that prepared by Tween 80 Protocol showed an effective vaccine candidate that protected animals, eliminated the virulence in feces and liver. These findings show that chemically induced bacterial ghosts of Salmonella Typhimurium can be a promising vaccine.
Identifiants
pubmed: 35180858
doi: 10.1186/s12917-021-03112-4
pii: 10.1186/s12917-021-03112-4
pmc: PMC8855557
doi:
Substances chimiques
Antibodies, Bacterial
0
Bacterial Vaccines
0
Salmonella Vaccines
0
Vaccines, Attenuated
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
72Informations de copyright
© 2022. The Author(s).
Références
Adv Exp Med Biol. 2009;655:159-70
pubmed: 20047041
Saudi Pharm J. 2018 Feb;26(2):232-237
pubmed: 30166921
Lab Anim (NY). 2011 May;40(5):155-60
pubmed: 21508954
BMC Immunol. 2014 Dec 31;15:584
pubmed: 25551828
Vet Immunol Immunopathol. 2015 Aug 15;166(3-4):138-44
pubmed: 26022514
ScientificWorldJournal. 2014 Mar 19;2014:840863
pubmed: 24772035
Expert Opin Biol Ther. 2001 Sep;1(5):765-71
pubmed: 11728212
ILAR J. 2002;43 Suppl:S31-8
pubmed: 12388849
J Microbiol Methods. 2010 Sep;82(3):334-7
pubmed: 20621134
J Biotechnol. 1996 Jan 26;44(1-3):161-70
pubmed: 8717400
Vaccine. 1994 Nov;12(14):1330-4
pubmed: 7856300
Infect Immun. 2005 Aug;73(8):4810-7
pubmed: 16040994
Foodborne Pathog Dis. 2017 Jan;14(1):1-7
pubmed: 27982711
Vaccine. 2003 Mar 28;21(13-14):1415-22
pubmed: 12615438
Vaccine. 2010 Aug 16;28(36):5760-7
pubmed: 20619379
Saudi Pharm J. 2014 Jul;22(3):273-9
pubmed: 25061413
ScientificWorldJournal. 2013;2013:545741
pubmed: 23576904
Microbes Infect. 2013 May;15(5):388-98
pubmed: 23485513
Vaccine. 2014 May 30;32(26):3249-55
pubmed: 24721534
Curr Opin Biotechnol. 2004 Dec;15(6):530-7
pubmed: 15560979
Bioeng Bugs. 2010 Sep-Oct;1(5):326-36
pubmed: 21326832
Infect Immun. 2015 Jul;83(7):2957-65
pubmed: 25964469
Int J Mol Sci. 2016 Nov 15;17(11):
pubmed: 27854308
Biotechnol Appl Biochem. 2004 Dec;40(Pt 3):209-28
pubmed: 15035661
Expert Opin Drug Deliv. 2006 Jan;3(1):11-22
pubmed: 16370937