Biotin
IL-6
Immunoassay
Negative interference
Journal
Annals of clinical and laboratory science
ISSN: 1550-8080
Titre abrégé: Ann Clin Lab Sci
Pays: United States
ID NLM: 0410247
Informations de publication
Date de publication:
Jan 2022
Jan 2022
Historique:
entrez:
19
2
2022
pubmed:
20
2
2022
medline:
26
2
2022
Statut:
ppublish
Résumé
Interleukin -6 (IL-6) is an important diagnostic test in COVID-19 patients to determine whether to initiate tocilizumab therapy or mechanical ventilation. We investigated potential interference of biotin in Roche IL-6 assay which utilizes biotinylated antibody. We prepared three serum pools from left-over specimens which showed IL-6 values over 40 pg/mL. Then aliquots of each serum pool were further supplemented with various amounts of biotin expected in patients taking biotin supplement and then IL-6 values were measured again using Roche IL-6 assay on the Cobas e411 analyzer. We observed negative interference of biotin in IL-6 assay but interference was bimodal as maximum negative interference was observed with 100 ng/mL biotin but not with 1000 ng/mL. However, no interference was observed in the presence of 25 ng/mL biotin. Biotin showed negative interference with IL-6 assay.
Substances chimiques
IL6 protein, human
0
Interleukin-6
0
Biotin
6SO6U10H04
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
161-163Informations de copyright
Copyright © 2022 by the Association of Clinical Scientists, Inc.