Bioconjugates of photon-upconversion nanoparticles for cancer biomarker detection and imaging.
Journal
Nature protocols
ISSN: 1750-2799
Titre abrégé: Nat Protoc
Pays: England
ID NLM: 101284307
Informations de publication
Date de publication:
04 2022
04 2022
Historique:
received:
04
05
2021
accepted:
19
11
2021
pubmed:
20
2
2022
medline:
12
4
2022
entrez:
19
2
2022
Statut:
ppublish
Résumé
The detection of cancer biomarkers in histological samples and blood is of paramount importance for clinical diagnosis. Current methods are limited in terms of sensitivity, hindering early detection of disease. We have overcome the shortcomings of currently available staining and fluorescence labeling methods by taking an integrative approach to establish photon-upconversion nanoparticles (UCNP) as a powerful platform for cancer detection. These nanoparticles are readily synthesized in different sizes to yield efficient and tunable short-wavelength light emission under near-infrared excitation, which eliminates optical background interference of the specimen. Here we present a protocol for the synthesis of UCNPs by high-temperature co-precipitation or seed-mediated growth by thermal decomposition, surface modification by silica or poly(ethylene glycol) that renders the particles resistant to nonspecific binding, and the conjugation of streptavidin or antibodies for biological detection. To detect blood-based biomarkers, we present an upconversion-linked immunosorbent assay for the analog and digital detection of the cancer marker prostate-specific antigen. When applied to immunocytochemistry analysis, UCNPs enable the detection of the breast cancer marker human epidermal growth factor receptor 2 with a signal-to-background ratio 50-fold higher than conventional fluorescent labels. UCNP synthesis takes 4.5 d, the preparation of the antibody-silica-UCNP conjugate takes 3 d, the streptavidin-poly(ethylene glycol)-UCNP conjugate takes 2-3 weeks, upconversion-linked immunosorbent assay takes 2-4 d and immunocytochemistry takes 8-10 h. The procedures can be performed after standard laboratory training in nanomaterials research.
Identifiants
pubmed: 35181766
doi: 10.1038/s41596-021-00670-7
pii: 10.1038/s41596-021-00670-7
doi:
Substances chimiques
Biomarkers, Tumor
0
Immunosorbents
0
Polyethylene Glycols
3WJQ0SDW1A
Silicon Dioxide
7631-86-9
Streptavidin
9013-20-1
Types de publication
Journal Article
Review
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1028-1072Subventions
Organisme : Deutsche Forschungsgemeinschaft (German Research Foundation)
ID : GO 1968/ 7-1 (Heisenberg Program)
Organisme : Deutsche Forschungsgemeinschaft (German Research Foundation)
ID : GO 1968/6-1
Organisme : Grantová Agentura České Republiky (Grant Agency of the Czech Republic)
ID : 21-03156S
Organisme : Grantová Agentura České Republiky (Grant Agency of the Czech Republic)
ID : 21-04420S
Organisme : Ministerstvo Školství, Mládeže a Tělovýchovy (Ministry of Education, Youth and Sports)
ID : LTAB19011
Organisme : Ministerstvo Školství, Mládeže a Tělovýchovy (Ministry of Education, Youth and Sports)
ID : LQ1601
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer Nature Limited.
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