Extracellular vesicles released after cranial radiation: An insight into an early mechanism of brain injury.
Extracellular vesicles
Lipid peroxidation
Oxidative stress
Radiation
Journal
Brain research
ISSN: 1872-6240
Titre abrégé: Brain Res
Pays: Netherlands
ID NLM: 0045503
Informations de publication
Date de publication:
01 05 2022
01 05 2022
Historique:
received:
20
10
2021
revised:
12
02
2022
accepted:
15
02
2022
pubmed:
21
2
2022
medline:
26
4
2022
entrez:
20
2
2022
Statut:
ppublish
Résumé
Cranial radiation is important for treating both primary brain tumors and brain metastases. A potential delayed side effect of cranial radiation is neurocognitive function decline. Early detection of CNS injury might prevent further neuronal damage. Extracellular vesicles (EVs) have emerged as a potential diagnostic tool because of their unique membranous characteristics and cargos. We investigated whether EVs can be an early indicator of CNS injury by giving C57BJ/6 mice 10 Gy cranial IR. EVs were isolated from sera to quantify: 1) number of EVs using nanoparticle tracking analysis (NTA); 2) Glial fibrillary acidic protein (GFAP), an astrocyte marker; and 3) protein-bound 4-hydroxy-2-nonenal (HNE) adducts, an oxidative damage marker. Brain tissues were prepared for immunohistochemistry staining and protein immunoblotting. The results demonstrate: 1) increased GFAP levels (p < 0.05) in EVs, but not brain tissue, in the IR group; and 2) increased HNE-bound protein adduction levels (p < 0.05). The results support using EVs as an early indicator of cancer therapy-induced neuronal injury.
Identifiants
pubmed: 35183524
pii: S0006-8993(22)00064-6
doi: 10.1016/j.brainres.2022.147840
pmc: PMC8918058
mid: NIHMS1784491
pii:
doi:
Substances chimiques
Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
147840Subventions
Organisme : NIGMS NIH HHS
ID : P20 GM121327
Pays : United States
Organisme : NIEHS NIH HHS
ID : T32 ES007266
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA217934
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA177558
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR001998
Pays : United States
Informations de copyright
Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.
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