Essential trace and toxic elemental concentrations in biological samples of male adult referent and Eunuch subjects.


Journal

Clinica chimica acta; international journal of clinical chemistry
ISSN: 1873-3492
Titre abrégé: Clin Chim Acta
Pays: Netherlands
ID NLM: 1302422

Informations de publication

Date de publication:
01 Apr 2022
Historique:
received: 29 08 2021
revised: 14 02 2022
accepted: 14 02 2022
pubmed: 21 2 2022
medline: 18 3 2022
entrez: 20 2 2022
Statut: ppublish

Résumé

The influence of essential nutrients such as calcium (Ca), copper (Cu), iron (Fe), magnesium (Mg), selenium (Se), and zinc (Zn) on male fertility has been extensively studied. For reproductive health, adequate spermatogenesis, sperm maturation and motility, as well as optimal sperm function, these essential elements are required. Several pathologic and male infertility disorders have been linked to an imbalance of these elements. The purpose of this study was to determine the relationship between essential and toxic elements, such as Ca, Cu, Fe, Mg Se, and Zn, as well as cadmium (Cd), lead (Pb), and mercury (Hg) in human biological samples, such as blood, serum, and seminal plasma, from 96 referent male adults aged 21 to 49 years, who were further divided into three age groups. The biological samples of Eunuch people (n = 37), ranging in age from 21 to 42 years, were also analysed for comparison. The method of acid digestion was controlled by a microwave oven. The essential and toxic elements in the oxidised biological samples were determined using atomic absorption spectrometry. Certified reference material of blood and serum was being used to validate the methodology's accuracy. The Ca, Cu, Fe, Mg, Se, and Zn concentrations in blood and serum samples of Eunuch people were found to be lower, whereas Cd, Pb, and Hg levels were significantly higher than those found in data from normal adult male subjects (p 0.001). The results reveal that the concentrations of Ca, Cu, Fe, Mg, Se, and Zn in the seminal plasma of adult male participants in the younger age group (21-29) were higher than those in the older age groups (30-39) and (40-49). Although essential trace element levels in Eunuch people's biological samples were significantly lower than those observed in adult male subjects. Essential elemental deficiency and elevated levels of toxic heavy metals in all biological fluid samples may have significant negative effects on human reproductive health (sperm quality and function), leading to male infertility.

Sections du résumé

BACKGROUND BACKGROUND
The influence of essential nutrients such as calcium (Ca), copper (Cu), iron (Fe), magnesium (Mg), selenium (Se), and zinc (Zn) on male fertility has been extensively studied. For reproductive health, adequate spermatogenesis, sperm maturation and motility, as well as optimal sperm function, these essential elements are required. Several pathologic and male infertility disorders have been linked to an imbalance of these elements.
METHODS METHODS
The purpose of this study was to determine the relationship between essential and toxic elements, such as Ca, Cu, Fe, Mg Se, and Zn, as well as cadmium (Cd), lead (Pb), and mercury (Hg) in human biological samples, such as blood, serum, and seminal plasma, from 96 referent male adults aged 21 to 49 years, who were further divided into three age groups. The biological samples of Eunuch people (n = 37), ranging in age from 21 to 42 years, were also analysed for comparison. The method of acid digestion was controlled by a microwave oven. The essential and toxic elements in the oxidised biological samples were determined using atomic absorption spectrometry. Certified reference material of blood and serum was being used to validate the methodology's accuracy.
RESULT RESULTS
The Ca, Cu, Fe, Mg, Se, and Zn concentrations in blood and serum samples of Eunuch people were found to be lower, whereas Cd, Pb, and Hg levels were significantly higher than those found in data from normal adult male subjects (p 0.001). The results reveal that the concentrations of Ca, Cu, Fe, Mg, Se, and Zn in the seminal plasma of adult male participants in the younger age group (21-29) were higher than those in the older age groups (30-39) and (40-49). Although essential trace element levels in Eunuch people's biological samples were significantly lower than those observed in adult male subjects.
CONCLUSION CONCLUSIONS
Essential elemental deficiency and elevated levels of toxic heavy metals in all biological fluid samples may have significant negative effects on human reproductive health (sperm quality and function), leading to male infertility.

Identifiants

pubmed: 35183529
pii: S0009-8981(22)00060-2
doi: 10.1016/j.cca.2022.02.010
pii:
doi:

Substances chimiques

Trace Elements 0
Copper 789U1901C5
Selenium H6241UJ22B
Zinc J41CSQ7QDS

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

96-103

Informations de copyright

Copyright © 2022 Elsevier B.V. All rights reserved.

Auteurs

Hassan Imran Afridi (H)

National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, Pakistan. Electronic address: hassan.afridi@usindh.edu.pk.

Tasneem Gul Kazi (T)

National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, Pakistan. Electronic address: tasneem.kazi@usindh.edu.pk.

Farah Naz Talpur (F)

National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, Pakistan. Electronic address: farah.talpur@usindh.edu.pk.

Jameel Ahmed Baig (J)

National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, Pakistan. Electronic address: jameel.baig@usindh.edu.pk.

Ghulam Qadir Chanihoon (G)

National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, Pakistan. Electronic address: qadir.soomar@scholars.usindh.edu.pk.

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Classifications MeSH