The Histone H3K27me3 Demethylases KDM6A/B Resist Anoikis and Transcriptionally Regulate Stemness-Related Genes.

CD44 HIF1α SOX2 anoikis histone demethylases

Journal

Frontiers in cell and developmental biology
ISSN: 2296-634X
Titre abrégé: Front Cell Dev Biol
Pays: Switzerland
ID NLM: 101630250

Informations de publication

Date de publication:
2022
Historique:
received: 20 09 2021
accepted: 10 01 2022
entrez: 21 2 2022
pubmed: 22 2 2022
medline: 22 2 2022
Statut: epublish

Résumé

Epithelial cancer cells that lose attachment from the extracellular matrix (ECM) to seed in a distant organ often undergo anoikis's specialized form of apoptosis. Recently, KDM3A (H3K9 demethylase) has been identified as a critical effector of anoikis in cancer cells. However, whether other histone demethylases are involved in promoting or resisting anoikis remains elusive. We screened the major histone demethylases and found that both H3K27 histone demethylases, namely, KDM6A/B were highly expressed during ECM detachment. Inhibition of the KDM6A/B activity by using a specific inhibitor results in reduced sphere formation capacity and increased apoptosis. Knockout of KDM6B leads to the loss of stem cell properties in solitary cells. Furthermore, we found that KDM6B maintains stemness by transcriptionally regulating the expression of stemness genes

Identifiants

pubmed: 35186918
doi: 10.3389/fcell.2022.780176
pii: 780176
pmc: PMC8847600
doi:

Types de publication

Journal Article

Langues

eng

Pagination

780176

Informations de copyright

Copyright © 2022 Shait Mohammed, Zamzami, Choudhry, Ahmed, Ateeq and Khan.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Auteurs

Mohammed Razeeth Shait Mohammed (MR)

Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
Centre of Artificial Intelligence in Precision Medicines, King Abdulaziz University, Jeddah, Saudi Arabia.

Mazin Zamzami (M)

Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.

Hani Choudhry (H)

Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
Centre of Artificial Intelligence in Precision Medicines, King Abdulaziz University, Jeddah, Saudi Arabia.

Firoz Ahmed (F)

Department of Biochemistry, College of Science, University of Jeddah, Jeddah, Saudi Arabia.
University of Jeddah Centre for Scientific and Medical Research (UJ-`CSMR), University of Jeddah, Jeddah, Saudi Arabia.

Bushra Ateeq (B)

Molecular Oncology Lab, Department of Biological Sciences and Bioengineering, Indian Institute of Technology-Kanpur (IIT-K), Kanpur, India.

Mohammad Imran Khan (MI)

Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
Centre of Artificial Intelligence in Precision Medicines, King Abdulaziz University, Jeddah, Saudi Arabia.

Classifications MeSH