Isochoric supercooling cryomicroscopy.
Cryomicroscopy
Cryopreservation
HeLa cells
Isochoric
Supercooling
Journal
Cryobiology
ISSN: 1090-2392
Titre abrégé: Cryobiology
Pays: Netherlands
ID NLM: 0006252
Informations de publication
Date de publication:
06 2022
06 2022
Historique:
received:
24
10
2021
revised:
09
02
2022
accepted:
09
02
2022
pubmed:
22
2
2022
medline:
9
6
2022
entrez:
21
2
2022
Statut:
ppublish
Résumé
We introduce an isochoric (constant-volume) supercooling cryomicroscope (ISCM), enabling the ice-free study of biological systems and biochemical reactions at subzero temperatures at atmospheric pressure absent ice. This technology draws from thermodynamic findings on the behavior of water in isochoric systems at subfreezing temperatures. A description of the design of the ISCM and a demonstration of the stability of the supercooled solution in the ISCM is followed by an illustration of the possible use of the ISCM in the preservation of biological matter research. A comparison was made between the survival of HeLa cells in the University of Wisconsin (UW) solution in the ISCM at +4 °C under conventional atmospheric conditions and at -5 °C under isochoric supercooled conditions. Continuous real-time monitoring at cryopreservation temperature via fluorescence microscopy showed that after three days of isochoric supercooling storage, the percentage of compromised cells remained similar to fresh controls, while storage at +4 °C yielded approximately three times the mortality rate of cells preserved at -5 °C.
Identifiants
pubmed: 35189096
pii: S0011-2240(22)00018-9
doi: 10.1016/j.cryobiol.2022.02.002
pii:
doi:
Substances chimiques
Isochores
0
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
139-147Informations de copyright
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