Panel of human cell lines with human/mouse artificial chromosomes.

Animals Cell Line Chromosomes, Artificial, Human Gene Transfer Techniques Genetic Vectors HEK293 Cells Humans Induced Pluripotent Stem Cells Mesenchymal Stem Cells Mice Molecular Biology

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
22 02 2022
Historique:
received: 08 07 2021
accepted: 04 02 2022
entrez: 23 2 2022
pubmed: 24 2 2022
medline: 18 3 2022
Statut: epublish

Résumé

Human artificial chromosomes (HACs) and mouse artificial chromosomes (MACs) are non-integrating chromosomal gene delivery vectors for molecular biology research. Recently, microcell-mediated chromosome transfer (MMCT) of HACs/MACs has been achieved in various human cells that include human immortalised mesenchymal stem cells (hiMSCs) and human induced pluripotent stem cells (hiPSCs). However, the conventional strategy of gene introduction with HACs/MACs requires laborious and time-consuming stepwise isolation of clones for gene loading into HACs/MACs in donor cell lines (CHO and A9) and then transferring the HAC/MAC into cells via MMCT. To overcome these limitations and accelerate chromosome vector-based functional assays in human cells, we established various human cell lines (HEK293, HT1080, hiMSCs, and hiPSCs) with HACs/MACs that harbour a gene-loading site via MMCT. Model genes, such as tdTomato, TagBFP2, and ELuc, were introduced into these preprepared HAC/MAC-introduced cell lines via the Cre-loxP system or simultaneous insertion of multiple gene-loading vectors. The model genes on the HACs/MACs were stably expressed and the HACs/MACs were stably maintained in the cell lines. Thus, our strategy using this HAC/MAC-containing cell line panel has dramatically simplified and accelerated gene introduction via HACs/MACs.

Identifiants

DOI: 10.1038/s41598-022-06814-3 PMID: 35194085 PMC: PMC8863800
pubmed: 35194085
doi: 10.1038/s41598-022-06814-3
pii: 10.1038/s41598-022-06814-3
pmc: PMC8863800
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

3009

Informations de copyright

© 2022. The Author(s).

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Auteurs

Narumi Uno (N)

Division of Genome and Cellular Functions, Department of Molecular and Cellular Biology, Faculty of Medicine, School of Life Science, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan. narumi@toyaku.ac.jp.
Chromosome Engineering Research Center, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan. narumi@toyaku.ac.jp.
Laboratory of Bioengineering, Faculty of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachiohji, Tokyo, 192-0392, Japan. narumi@toyaku.ac.jp.

Shuta Takata (S)

Division of Genome and Cellular Functions, Department of Molecular and Cellular Biology, Faculty of Medicine, School of Life Science, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan.

Shinya Komoto (S)

Division of Genome and Cellular Functions, Department of Molecular and Cellular Biology, Faculty of Medicine, School of Life Science, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan.

Hitomaru Miyamoto (H)

Division of Genome and Cellular Functions, Department of Molecular and Cellular Biology, Faculty of Medicine, School of Life Science, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan.

Yuji Nakayama (Y)

Division of Radioisotope Science, Research Initiative Center, Organization for Research Initiative and Promotion, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan.

Mitsuhiko Osaki (M)

Chromosome Engineering Research Center, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan.
Division of Experimental Pathology, Department of Biomedical Sciences, Faculty of Medicine, Tottori University, Yonago, Tottori, 683-8503, Japan.

Ryota Mayuzumi (R)

Laboratory of Bioengineering, Faculty of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachiohji, Tokyo, 192-0392, Japan.

Natsumi Miyazaki (N)

Laboratory of Bioengineering, Faculty of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachiohji, Tokyo, 192-0392, Japan.

Chiaki Hando (C)

Laboratory of Bioengineering, Faculty of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachiohji, Tokyo, 192-0392, Japan.

Satoshi Abe (S)

Chromosome Engineering Research Center, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan.

Tetsushi Sakuma (T)

Division of Integrated Sciences for Life, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Hiroshima, 739-8526, Japan.

Takashi Yamamoto (T)

Division of Integrated Sciences for Life, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Hiroshima, 739-8526, Japan.

Teruhiko Suzuki (T)

Stem Cell Project, Tokyo Metropolitan Institute of Medical Science, Kamikitazawa, Setagaya-ku, Tokyo, 156-8506, Japan.

Yoshihiro Nakajima (Y)

Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu, Kagawa, 761-0395, Japan.

Mitsuo Oshimura (M)

Chromosome Engineering Research Center, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan.

Kazuma Tomizuka (K)

Laboratory of Bioengineering, Faculty of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachiohji, Tokyo, 192-0392, Japan.

Yasuhiro Kazuki (Y)

Division of Genome and Cellular Functions, Department of Molecular and Cellular Biology, Faculty of Medicine, School of Life Science, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan. kazuki@tottori-u.ac.jp.
Chromosome Engineering Research Center, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan. kazuki@tottori-u.ac.jp.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Panel of human cell lines with human/mouse...
questionsmedicales.fr › Cellules › Cellules cultivées › Lignée cellulaire › Panel of human cell lines with human/mouse...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Vecteurs génétiques › Chromosomes artificiels › Chromosomes artificiels de mammifère › Chromosomes artificiels humains › Panel of human cell lines with human/mouse...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques de transfert de gènes › Panel of human cell lines with human/mouse...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Vecteurs génétiques › Panel of human cell lines with human/mouse...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules HEK293 › Panel of human cell lines with human/mouse...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Panel of human cell lines with human/mouse...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches pluripotentes › Cellules souches pluripotentes induites › Panel of human cell lines with human/mouse...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches multipotentes › Cellules souches mésenchymateuses › Panel of human cell lines with human/mouse...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Panel of human cell lines with human/mouse...
questionsmedicales.fr › Disciplines des sciences naturelles › Chimie › Biochimie › Biologie moléculaire › Panel of human cell lines with human/mouse...