Analgesic and antiinflammatory effects of Nigella orientalis L. seeds fixed oil: Pharmacological potentials and molecular mechanisms.
Nigella orientalis
RAW 264.7 cells
analgesic activity
antiinflammatory
lipopolysaccharide (LPS)
signaling pathways
Journal
Phytotherapy research : PTR
ISSN: 1099-1573
Titre abrégé: Phytother Res
Pays: England
ID NLM: 8904486
Informations de publication
Date de publication:
Mar 2022
Mar 2022
Historique:
revised:
17
01
2022
received:
13
10
2021
accepted:
17
01
2022
pubmed:
24
2
2022
medline:
26
3
2022
entrez:
23
2
2022
Statut:
ppublish
Résumé
Nigella species have been widely used in traditional medicine. The aim of this study was to evaluate the antiinflammatory and analgesic potentials of Nigella orientalis L. seeds fixed oil (NOO). The acetic acid writhing test and the formaldehyde-induced licking paw were performed to assess the analgesic activity of the oil. The antiinflammatory activity was first evaluated in vitro by the erythrocyte membrane stabilization then in vivo by xylene- and carrageenan-induced ear and paw edema, respectively. To further understand the molecular mechanism of action of the Nigella extract, lipopolysaccharide-activated RAW 264.7 macrophages were used. Nitric oxide (NO) production was measured by Griess reaction and cell viability by MTT assay. The gene and protein expression of inflammatory mediators were assessed by RT-PCR and western blot, respectively. NOO exerted a potent analgesic effect in in vivo models of writhing test and induced edema. The analyzed molecular mechanisms revealed a role for NO and prostaglandins as molecules mediating the pharmacological effects of the extract through a mechanism involving nuclear factor-κB and mitogen-activated protein kinases. This study demonstrates, for the first time, that the fixed oil of N. orientalis has strong antinociceptive and antiinflammatory properties and might be a promising agent for the treatment of certain inflammation-related diseases.
Substances chimiques
Analgesics
0
Anti-Inflammatory Agents
0
Plant Extracts
0
Carrageenan
9000-07-1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1372-1385Subventions
Organisme : Algerian Ministry of Higher Education and Scientific Research for Exceptional National Program Grant
ID : PNE 2019/2020-No.115
Organisme : Consellería de Economía, Emprego e Industria, Xunta de Galicia
ID : GAIN GPC IN607B2019/10
Organisme : Instituto de Salud Carlos III
ID : PI17/00409
Organisme : Instituto de Salud Carlos III
ID : PI20/00902
Organisme : Instituto de Salud Carlos III
ID : PI18/00821
Organisme : Instituto de Salud Carlos III
ID : CB16/11/00226
Organisme : Instituto de Salud Carlos III
ID : RD16/0012/0014
Organisme : Instituto de Salud Carlos III
ID : RD21/0002/0025
Organisme : Research Executive Agency
ID : MSCA-RISE 734899
Organisme : Secretaría de Estado de Universidades, Investigación, Desarrollo e Innovación, Ministerio de Universidades
ID : FPU2018-04165
Organisme : Xunta de Galicia
ID : IN606A-2020/010
Organisme : Fondo Regional de Desarrollo Regional
ID : Exp.18/00188
Informations de copyright
© 2022 John Wiley & Sons Ltd.
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