Development of duplex qPCR targeting Carnobacterium maltaromaticum and Vagococcus salmoninarum.
Carnobacterium maltaromaticum
Vagococcus salmoninarum
coinfection
duplex qPCR
lake trout
Journal
Journal of fish diseases
ISSN: 1365-2761
Titre abrégé: J Fish Dis
Pays: England
ID NLM: 9881188
Informations de publication
Date de publication:
May 2022
May 2022
Historique:
revised:
21
01
2022
received:
01
12
2021
accepted:
22
01
2022
pubmed:
24
2
2022
medline:
8
4
2022
entrez:
23
2
2022
Statut:
ppublish
Résumé
In November 2018, Vagococcus salmoninarum was identified as the causative agent of a chronic coldwater streptococcosis epizootic in broodstock brook trout (Salvelinus fontinalis) at the Iron River National Fish Hatchery in Wisconsin, USA. By February 2019, the epizootic spread to adjacent raceways containing broodstock lake trout (Salvelinus namaycush), whereby fish were found to be coinfected with Carnobacterium maltaromaticum and V. salmoninarum. To differentiate these two pathogens and determine the primary cause of the lake trout morbidity, a quantitative real-time PCR (qPCR) was developed targeting the C. maltaromaticum phenylalanyl-tRNA synthase alpha subunit (pheS) gene. The qPCR was combined with a V. salmoninarum qPCR, creating a duplex qPCR assay that simultaneously quantitates C. maltaromaticum and V. salmoninarum concentrations in individual lake trout tissues, and screens presumptive isolates from hatchery inspections and wild fish from national fish hatchery source waters throughout the Great Lakes basin. Vagococcus salmoninarum and C. maltaromaticum were co-detected in broodstock brook trout from two tribal hatcheries and C. maltaromaticum was present in wild fish in source waters of several national fish hatcheries. This study provides a powerful new tool to differentiate and diagnose two emerging Gram-positive bacterial pathogens.
Banques de données
RefSeq
['AM1684925', 'HE590726', 'HE590729', 'HE590732', 'HE590735', 'HE590741', 'HE590738', 'HE615154', 'AM694187', 'KY606577', 'MT893157', 'MK226201']
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
667-677Informations de copyright
© 2022 John Wiley & Sons Ltd. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.
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