Structural and biochemical characterization of the relaxosome auxiliary proteins encoded on the
AUC, Analytical Ultracentrifugation
Antibiotic resistance
Auxiliary protein
Bacterial conjugation
DNA binding protein
EDTA, Ethylenediaminetetraacetic acid
EM, Electron Micoscopy
Firmicutes
HGT, Horizontal Gene Transfer
Horizontal gene transfer
IPTG, Isopropyl β-D-1-thiogalactopyranoside
ITC, Isothermal titration calorimetry
MGE, Mobile Genetic Element
PMSF, phenylmethylsulfonyl fluoride
RHH, Ribbon-Helix-Helix
Relaxosome
Ribbon-Helix-Helix
SAXS, Small-angle X-ray scattering
SDS-PAGE, sodium dodecyl sulphate–polyacrylamide gel electrophoresis
SEC, Size Exclusion Chromatography
Structural biology
T4SS, Type IV secretion system
oriT, Origin of Transfer
Journal
Computational and structural biotechnology journal
ISSN: 2001-0370
Titre abrégé: Comput Struct Biotechnol J
Pays: Netherlands
ID NLM: 101585369
Informations de publication
Date de publication:
2022
2022
Historique:
received:
14
03
2021
revised:
22
12
2021
accepted:
30
12
2021
entrez:
24
2
2022
pubmed:
25
2
2022
medline:
25
2
2022
Statut:
epublish
Résumé
Bacterial conjugation is an important route for horizontal gene transfer. The initial step in this process involves a macromolecular protein-DNA complex called the relaxosome, which in plasmids consists of the origin of transfer (
Identifiants
pubmed: 35198129
doi: 10.1016/j.csbj.2021.12.041
pii: S2001-0370(22)00004-6
pmc: PMC8829557
doi:
Types de publication
Journal Article
Langues
eng
Pagination
757-765Informations de copyright
© 2022 The Authors.
Déclaration de conflit d'intérêts
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Références
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):235-42
pubmed: 21460441
Proc Natl Acad Sci U S A. 2013 Feb 19;110(8):2804-9
pubmed: 23359708
Mol Microbiol. 2021 Mar;115(3):436-452
pubmed: 33326642
Trends Microbiol. 2000 Aug;8(8):354-60
pubmed: 10920394
Mol Microbiol. 2008 Oct;70(1):89-99
pubmed: 18717787
Biochim Biophys Acta. 2014 Aug;1843(8):1578-91
pubmed: 24389247
FEMS Microbiol Rev. 2010 Jan;34(1):18-40
pubmed: 19919603
Plasmid. 2013 Nov;70(3):289-302
pubmed: 24129002
J Mol Biol. 2014 Nov 11;426(22):3783-3795
pubmed: 25284757
Acta Crystallogr D Biol Crystallogr. 2006 Jan;62(Pt 1):72-82
pubmed: 16369096
Curr Top Microbiol Immunol. 2017;413:1-30
pubmed: 29536353
Curr Opin Struct Biol. 2006 Dec;16(6):744-52
pubmed: 17079132
Plasmid. 2019 Jul;104:102415
pubmed: 31103521
Nucleic Acids Res. 2014 May;42(9):5715-27
pubmed: 24623814
Front Mol Biosci. 2016 Nov 10;3:71
pubmed: 27891505
Methods Mol Biol. 2020;2075:295-308
pubmed: 31584171
Nat Methods. 2013 Nov;10(11):1099-101
pubmed: 24037245
Mol Microbiol. 2018 Feb;107(4):455-471
pubmed: 29235173
Microbiol Mol Biol Rev. 2003 Jun;67(2):277-301, table of contents
pubmed: 12794193
EMBO J. 2006 Jun 21;25(12):2930-9
pubmed: 16710295
RSC Chem Biol. 2020 Aug 28;1(4):281-287
pubmed: 34458766
Front Microbiol. 2019 May 22;10:1134
pubmed: 31191478
Mol Microbiol. 2012 Aug;85(4):602-17
pubmed: 22788760
Philos Trans R Soc Lond B Biol Sci. 2012 Apr 19;367(1592):1073-87
pubmed: 22411979
Front Microbiol. 2017 Nov 30;8:2353
pubmed: 29250047
J Mol Recognit. 1999 Jan-Feb;12(1):3-18
pubmed: 10398392
Nucleic Acids Res. 2011 Aug;39(15):6775-88
pubmed: 21565799
Nucleic Acids Res. 2019 Jul 2;47(W1):W402-W407
pubmed: 31251384
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501
pubmed: 20383002
Front Microbiol. 2011 Jun 27;2:136
pubmed: 21743810
Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):1002-11
pubmed: 16929101
Proc Natl Acad Sci U S A. 2017 Aug 8;114(32):E6526-E6535
pubmed: 28739894
FEMS Microbiol Rev. 2014 Jul;38(4):720-60
pubmed: 24372381
Cell. 2017 May 4;169(4):708-721.e12
pubmed: 28457609
Microbiol Spectr. 2019 Mar;7(2):
pubmed: 30953428
Acta Crystallogr D Struct Biol. 2019 Oct 1;75(Pt 10):861-877
pubmed: 31588918
Front Microbiol. 2017 Nov 03;8:2138
pubmed: 29163424
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):125-32
pubmed: 20124692
Microbiol Mol Biol Rev. 2010 Sep;74(3):434-52
pubmed: 20805406
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):293-302
pubmed: 21460447
Acta Crystallogr D Biol Crystallogr. 2013 Jul;69(Pt 7):1204-14
pubmed: 23793146
Nature. 2014 Apr 24;508(7497):550-553
pubmed: 24670658
J Synchrotron Radiat. 2014 Jul;21(Pt 4):679-89
pubmed: 24971961
Antimicrob Agents Chemother. 2009 Feb;53(2):735-47
pubmed: 19029325
J Bacteriol. 2005 Nov;187(22):7727-37
pubmed: 16267297
J Synchrotron Radiat. 2019 Mar 1;26(Pt 2):393-405
pubmed: 30855248
Mol Microbiol. 2007 Feb;63(4):1173-84
pubmed: 17238924
PLoS Genet. 2017 Feb 16;13(2):e1006586
pubmed: 28207825
J Appl Crystallogr. 2009 Apr 1;42(Pt 2):342-346
pubmed: 27630371
Nature. 1994 Feb 24;367(6465):754-7
pubmed: 8107872
Nat Rev Microbiol. 2007 Sep;5(9):710-20
pubmed: 17676053
Microbiol Rev. 1994 Jun;58(2):162-210
pubmed: 7915817