Magneto-Immunoassay for the Detection and Quantification of Human Growth Hormone.
gold shell magnetic nanoparticles
human growth hormone
sandwich-type colorimetric magneto-immunoassay
Journal
Biosensors
ISSN: 2079-6374
Titre abrégé: Biosensors (Basel)
Pays: Switzerland
ID NLM: 101609191
Informations de publication
Date de publication:
25 Jan 2022
25 Jan 2022
Historique:
received:
19
12
2021
revised:
19
01
2022
accepted:
22
01
2022
entrez:
24
2
2022
pubmed:
25
2
2022
medline:
7
4
2022
Statut:
epublish
Résumé
Physiological and endocrine maintenance of a normal human growth hormone (hGH) concentration is crucial for growth, development, and a number of essential biological processes. In this study, we describe the preparation and characterization of magnetic nanoparticles coated with a gold shell (MNPs-Au). The optimal surface concentration of monoclonal anti-hGH antibodies (m-anti-hGH) on magnetic nanoparticles, as well as conditions that decrease non-specific interactions during the magneto-immunoassay, were elaborated. After the selective recognition, separation, and pre-concentration of hGH by MNPs-Au/m-anti-hGH and the hGH interaction with specific polyclonal biotin-labeled antibodies (p-anti-hHG-B) and streptavidin modified horseradish peroxidase (S-HRP), the MNPs-Au/m-anti-hGH/hGH/p-anti-hGH-B/S-HRP immunoconjugate was formed. The concentration of hGH was determined after the addition of 3,3',5,5'-tetramethylbenzidine and hydrogen peroxide substrate solution for HRP; the absorbance at 450 nm was registered after the addition of STOP solution. The developed sandwich-type colorimetric magneto-immunoassay is characterized by a clinically relevant linear range (from 0.1 to 5.0 nmol L
Identifiants
pubmed: 35200326
pii: bios12020065
doi: 10.3390/bios12020065
pmc: PMC8869458
pii:
doi:
Substances chimiques
Human Growth Hormone
12629-01-5
Gold
7440-57-5
Horseradish Peroxidase
EC 1.11.1.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : European Regional Development Fund through the Research Council of Lithuania according to the Programme Attracting Foreign Researchers for Research Implementation
ID : 01.2.2-LMT-K-718-02-0022.
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