Rubisco regulation in response to altered carbon status in the cyanobacterium Synechococcus elongatus PCC 7942.
Journal
Plant physiology
ISSN: 1532-2548
Titre abrégé: Plant Physiol
Pays: United States
ID NLM: 0401224
Informations de publication
Date de publication:
01 06 2022
01 06 2022
Historique:
accepted:
22
12
2021
received:
11
10
2021
pubmed:
25
2
2022
medline:
3
6
2022
entrez:
24
2
2022
Statut:
ppublish
Résumé
Photosynthetic organisms possess a variety of mechanisms to achieve balance between absorbed light (source) and the capacity to metabolically utilize or dissipate this energy (sink). While regulatory processes that detect changes in metabolic status/balance are relatively well studied in plants, analogous pathways remain poorly characterized in photosynthetic microbes. Here, we explored systemic changes that result from alterations in carbon availability in the model cyanobacterium Synechococcus elongatus PCC 7942 by taking advantage of an engineered strain where influx/efflux of a central carbon metabolite, sucrose, can be regulated experimentally. We observed that induction of a high-flux sucrose export pathway leads to depletion of internal carbon storage pools (glycogen) and concurrent increases in estimates of photosynthetic activity. Further, a proteome-wide analysis and fluorescence reporter-based analysis revealed that upregulated factors following the activation of the metabolic sink are concentrated on ribulose-1,5-bisphosphate carboxylase-oxygenase (Rubisco) and auxiliary modules involved in Rubisco maturation. Carboxysome number and Rubisco activity also increased following engagement of sucrose secretion. Conversely, reversing the flux of sucrose by feeding exogenous sucrose through the heterologous transporter resulted in increased glycogen pools, decreased Rubisco abundance, and carboxysome reorganization. Our data suggest that Rubisco activity and organization are key variables connected to regulatory pathways involved in metabolic balancing in cyanobacteria.
Identifiants
pubmed: 35201348
pii: 6535719
doi: 10.1093/plphys/kiac065
pmc: PMC9157067
doi:
Substances chimiques
Bacterial Proteins
0
Carbon Dioxide
142M471B3J
Sucrose
57-50-1
Carbon
7440-44-0
Glycogen
9005-79-2
Ribulose-Bisphosphate Carboxylase
EC 4.1.1.39
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
874-888Informations de copyright
© American Society of Plant Biologists 2022. All rights reserved. For permissions, please email: journals.permissions@oup.com.
Références
Proc Natl Acad Sci U S A. 2006 May 9;103(19):7246-51
pubmed: 16641091
Nat Rev Microbiol. 2008 Sep;6(9):681-91
pubmed: 18679172
J Exp Bot. 2016 Jan;67(1):31-45
pubmed: 26466662
J Exp Bot. 2011 Feb;62(4):1411-24
pubmed: 21282331
Plant Cell Physiol. 2017 Mar 1;58(3):537-545
pubmed: 28130420
Commun Biol. 2019 Sep 19;2:347
pubmed: 31552300
Annu Rev Plant Biol. 2002;53:449-75
pubmed: 12221984
Science. 2004 Jul 16;305(5682):367-71
pubmed: 15256665
Curr Microbiol. 2003 Jan;46(1):70-6
pubmed: 12432468
Photosynth Res. 2017 Sep;133(1-3):63-73
pubmed: 28283890
Proc Natl Acad Sci U S A. 2013 Jan 22;110(4):1249-54
pubmed: 23297225
J Vis Exp. 2018 Sep 11;(139):
pubmed: 30272659
Cell. 2006 Nov 3;127(3):579-89
pubmed: 17081979
Appl Environ Microbiol. 2010 May;76(10):3153-9
pubmed: 20363800
Appl Environ Microbiol. 2012 Apr;78(8):2660-8
pubmed: 22307292
J Plant Physiol. 2015 May 1;179:113-21
pubmed: 25863283
J Exp Bot. 2020 Mar 25;71(6):2005-2017
pubmed: 31858138
Arch Microbiol. 2003 Dec;180(6):471-83
pubmed: 14605795
Int J Mol Sci. 2018 Aug 24;19(9):
pubmed: 30149541
Metab Eng. 2016 Jul;36:28-36
pubmed: 26979472
FEMS Microbiol Lett. 2017 Sep 1;364(16):
pubmed: 28873946
Curr Opin Chem Biol. 2017 Dec;41:12-19
pubmed: 28968542
Plant Physiol. 2016 May;171(1):530-41
pubmed: 26956667
Sci Rep. 2020 Jan 15;10(1):390
pubmed: 31942010
Curr Opin Plant Biol. 2017 Jun;37:24-33
pubmed: 28391049
Trends Biochem Sci. 2016 Jan;41(1):62-76
pubmed: 26422689
Curr Opin Microbiol. 2021 Jun;61:58-66
pubmed: 33798818
Front Plant Sci. 2014 Jan 21;5:7
pubmed: 24478787
J Plant Physiol. 2004 Jun;161(6):715-24
pubmed: 15266719
Front Bioeng Biotechnol. 2014 Jun 19;2:21
pubmed: 25152894
Appl Environ Microbiol. 2018 Jan 2;84(2):
pubmed: 29101204
Science. 2010 Mar 5;327(5970):1258-61
pubmed: 20203050
J Bacteriol. 2011 Aug;193(15):3702-9
pubmed: 21642457
Photosynth Res. 2014 Jun;120(3):301-10
pubmed: 24590366
Mol Microbiol. 2017 Apr;104(2):260-277
pubmed: 28106321
Nature. 2019 Feb;566(7742):131-135
pubmed: 30675061
Methods Mol Biol. 2007;362:155-71
pubmed: 17417008
Nat Plants. 2016 Dec 02;2(12):16180
pubmed: 27909300
Plants (Basel). 2020 Mar 01;9(3):
pubmed: 32121540
Life (Basel). 2020 Nov 26;10(12):
pubmed: 33256109
J Biotechnol. 2020 Jun 20;317:1-4
pubmed: 32311395
Cell. 2013 Nov 21;155(5):1131-40
pubmed: 24267892
mSphere. 2018 Jan 24;3(1):
pubmed: 29404416
Proc Natl Acad Sci U S A. 2016 Aug 16;113(33):E4867-76
pubmed: 27486247
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Cell Rep. 2018 Oct 9;25(2):478-486.e8
pubmed: 30304686
Curr Protein Pept Sci. 2014;15(4):351-62
pubmed: 24678670
Nanoscale. 2017 Aug 3;9(30):10662-10673
pubmed: 28616951
Physiol Plant. 2008 Sep;134(1):216-26
pubmed: 18435694
Front Plant Sci. 2013 Jul 24;4:272
pubmed: 23898339
Nat Microbiol. 2016 Jun 20;1(7):16077
pubmed: 27572972
Front Bioeng Biotechnol. 2019 Feb 27;7:33
pubmed: 30873404
Plant Physiol. 2004 Oct;136(2):3301-12
pubmed: 15466225
Front Bioeng Biotechnol. 2014 Jul 01;2:22
pubmed: 25023122
Microbiol Mol Biol Rev. 1998 Sep;62(3):667-83
pubmed: 9729605
J Exp Bot. 2000 Apr;51(345):659-68
pubmed: 10938857
Plant Cell. 2019 Jul;31(7):1648-1664
pubmed: 31048338
Plant Cell Physiol. 2016 Dec;57(12):2451-2460
pubmed: 27742883
Proc Natl Acad Sci U S A. 2015 Apr 14;112(15):E1916-25
pubmed: 25825710
Plant Cell Physiol. 2016 Oct;57(10):2232-2243
pubmed: 27638927
ACS Synth Biol. 2021 Jul 16;10(7):1667-1681
pubmed: 34232633
Plant Physiol. 1998 Jul;117(3):1059-69
pubmed: 9662549
Annu Rev Plant Biol. 2006;57:675-709
pubmed: 16669778
J Plant Res. 2012 Jan;125(1):11-39
pubmed: 22006212
Nucleic Acids Res. 2019 Jan 8;47(D1):D442-D450
pubmed: 30395289
Plant Physiol. 2021 May 27;186(1):569-580
pubmed: 33576804
Elife. 2018 Dec 06;7:
pubmed: 30520729
Elife. 2014 Apr 29;:e02043
pubmed: 24842993
Proc Natl Acad Sci U S A. 2013 Mar 5;110(10):4111-6
pubmed: 23431195
J Exp Bot. 2003 Feb;54(383):609-22
pubmed: 12554704
PLoS One. 2013 Sep 04;8(9):e76127
pubmed: 24023971
Photochem Photobiol. 2008 Nov-Dec;84(6):1410-20
pubmed: 19067963
Plant Physiol. 2003 Dec;133(4):2069-80
pubmed: 14645730
Cell Rep. 2018 Apr 17;23(3):667-672
pubmed: 29669272
mBio. 2021 Aug 31;12(4):e0269620
pubmed: 34340540
Proc Natl Acad Sci U S A. 2014 Jun 10;111(23):8488-93
pubmed: 24889604
Sci Adv. 2020 May 06;6(19):eaba1269
pubmed: 32494723
J Exp Bot. 2009;60(2):357-64
pubmed: 19050062
Photosynth Res. 2013 Nov;117(1-3):31-44
pubmed: 23695654
Plant Cell. 2019 Mar;31(3):579-601
pubmed: 30787178
Environ Microbiol. 2009 Apr;11(4):927-36
pubmed: 19077009
Front Plant Sci. 2013 Mar 12;4:44
pubmed: 23487525
Photochem Photobiol Sci. 2020 May 1;19(5):585-603
pubmed: 32163064
Proc Natl Acad Sci U S A. 2021 Mar 16;118(11):
pubmed: 33836593