Analysis of the Glucose-Dependent Transcriptome in Murine Hypothalamic Cells.
RNA-seq
cAMP
cholesterol
glucose
hypothalamus
insulin-like growth factor
noradrenalin
serum response element
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
11 02 2022
11 02 2022
Historique:
received:
18
01
2022
revised:
10
02
2022
accepted:
10
02
2022
entrez:
25
2
2022
pubmed:
26
2
2022
medline:
9
4
2022
Statut:
epublish
Résumé
Glucose provides vital energy for cells and contributes to gene expression. The hypothalamus is key for metabolic homeostasis, but effects of glucose on hypothalamic gene expression have not yet been investigated in detail. Thus, herein, we monitored the glucose-dependent transcriptome in murine hypothalamic mHypoA-2/10 cells by total RNA-seq analysis. A total of 831 genes were up- and 1390 genes were downregulated by at least 50%. Key genes involved in the cholesterol biosynthesis pathway were upregulated, and total cellular cholesterol levels were significantly increased by glucose. Analysis of single genes involved in fundamental cellular signaling processes also suggested a significant impact of glucose. Thus, we chose ≈100 genes involved in signaling and validated the effects of glucose on mRNA levels by qRT-PCR. We identified
Identifiants
pubmed: 35203289
pii: cells11040639
doi: 10.3390/cells11040639
pmc: PMC8870115
pii:
doi:
Substances chimiques
Gnai1 protein, mouse
0
Cholesterol
97C5T2UQ7J
GTP-Binding Protein alpha Subunits, Gi-Go
EC 3.6.5.1
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : GRK 2338 RTG
Organisme : Ludwig-Maximilians-Universität München
ID : FöFoLe 25/2019
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