Digital Holographic Microscopy for Label-Free Detection of Leukocyte Alternations Associated with Perioperative Inflammation after Cardiac Surgery.
CPB
cardiac surgery
digital holographic microscopy
epinephrine
flow cytometry
label free
quantitative phase imaging
systemic inflammation
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
21 02 2022
21 02 2022
Historique:
received:
12
12
2021
revised:
16
02
2022
accepted:
19
02
2022
entrez:
25
2
2022
pubmed:
26
2
2022
medline:
9
4
2022
Statut:
epublish
Résumé
In a prospective observational pilot study on patients undergoing elective cardiac surgery with cardiopulmonary bypass, we evaluated label-free quantitative phase imaging (QPI) with digital holographic microscopy (DHM) to describe perioperative inflammation by changes in biophysical cell properties of lymphocytes and monocytes. Blood samples from 25 patients were investigated prior to cardiac surgery and postoperatively at day 1, 3 and 6. Biophysical and morphological cell parameters accessible with DHM, such as cell volume, refractive index, dry mass, and cell shape related form factor, were acquired and compared to common flow cytometric blood cell markers of inflammation and selected routine laboratory parameters. In all examined patients, cardiac surgery induced an acute inflammatory response as indicated by changes in routine laboratory parameters and flow cytometric cell markers. DHM results were associated with routine laboratory and flow cytometric data and correlated with complications in the postoperative course. In a subgroup analysis, patients were classified according to the inflammation related C-reactive protein (CRP) level, treatment with epinephrine and the occurrence of postoperative complications. Patients with regular courses, without epinephrine treatment and with low CRP values showed a postoperative lymphocyte volume increase. In contrast, the group of patients with increased CRP levels indicated an even further enlarged lymphocyte volume, while for the groups of epinephrine treated patients and patients with complicative courses, no postoperative lymphocyte volume changes were detected. In summary, the study demonstrates the capability of DHM to describe biophysical cell parameters of perioperative lymphocytes and monocytes changes in cardiac surgery patients. The pattern of correlations between biophysical DHM data and laboratory parameters, flow cytometric cell markers, and the postoperative course exemplify DHM as a promising diagnostic tool for a characterization of inflammatory processes and course of disease.
Identifiants
pubmed: 35203403
pii: cells11040755
doi: 10.3390/cells11040755
pmc: PMC8869820
pii:
doi:
Substances chimiques
Epinephrine
YKH834O4BH
Types de publication
Journal Article
Observational Study
Langues
eng
Sous-ensembles de citation
IM
Références
Chest. 2017 Sep;152(3):518-526
pubmed: 28625579
Blood. 1998 May 1;91(9):3439-46
pubmed: 9558403
Compr Physiol. 2015 Jan;5(1):1-15
pubmed: 25589262
Physiol Rev. 2009 Jan;89(1):193-277
pubmed: 19126758
J Trauma Acute Care Surg. 2012 May;72(5):1140-9
pubmed: 22673238
Perfusion. 2016 Jul;31(5):391-400
pubmed: 26503949
Nature. 1952 Mar 1;169(4296):366-7
pubmed: 14919571
Opt Express. 2015 Apr 6;23(7):8773-87
pubmed: 25968715
Ann Thorac Surg. 2015 Nov;100(5):1570-5; discussion 1575-6
pubmed: 26321440
J Clin Med. 2019 Mar 12;8(3):
pubmed: 30871101
PLoS One. 2021 May 21;16(5):e0252209
pubmed: 34019579
J Biomed Opt. 2013 Mar;18(3):036007
pubmed: 23487181
Int J Lab Hematol. 2014 Feb;36(1):e11-4
pubmed: 23795566
Int J Surg. 2005;3(2):129-40
pubmed: 17462274
J Biomed Opt. 2015 Nov;20(11):111218
pubmed: 26502322
J Cell Mol Med. 2020 Apr;24(8):4791-4803
pubmed: 32180339
J Struct Biol. 2012 Jun;178(3):270-8
pubmed: 22465356
Europace. 2017 Jan;19(1):40-47
pubmed: 26826137
J Clin Invest. 1990 Feb;85(2):462-7
pubmed: 2153706
Crit Care Med. 2016 Jan;44(1):218-28
pubmed: 26296143
Sci Rep. 2017 Jul 27;7(1):6654
pubmed: 28751719
Sci Rep. 2020 Jan 22;10(1):947
pubmed: 31969629
J Immunol. 2010 Jan 1;184(1):503-11
pubmed: 19949113
EJIFCC. 2019 Nov 25;30(4):371-384
pubmed: 31814812
Lab Chip. 2015 Aug 21;15(16):3278-85
pubmed: 26168054
Strahlenther Onkol. 2012 Nov;188(11):1038-47
pubmed: 23053158
Opt Lett. 2005 Mar 1;30(5):468-70
pubmed: 15789705
Am J Respir Crit Care Med. 2017 Apr 1;195(7):906-911
pubmed: 27649072
J Hematother Stem Cell Res. 2003 Jun;12(3):321-30
pubmed: 12857373
Opt Express. 2016 Feb 22;24(4):3177-88
pubmed: 26906982
Sci Rep. 2018 Jun 25;8(1):9624
pubmed: 29941960
Lab Chip. 2016 Feb 21;16(4):634-44
pubmed: 26732872
Blood Res. 2013 Sep;48(3):193-7
pubmed: 24086939
Integr Biol (Camb). 2012 Mar;4(3):280-4
pubmed: 22262315
Adv Exp Med Biol. 2020;1248:7-32
pubmed: 32185705
J Biophotonics. 2019 Sep;12(9):e201900085
pubmed: 31169960
J Biomed Opt. 2006 May-Jun;11(3):34005
pubmed: 16822055
Adv Sci (Weinh). 2018 Oct 11;5(12):1800761
pubmed: 30581697
Arch Pathol Lab Med. 2006 Mar;130(3):378-80
pubmed: 16519568
Cytometry A. 2021 May;99(5):511-523
pubmed: 32910546
Cytometry B Clin Cytom. 2021 Jan;100(1):103-114
pubmed: 33432735
Front Immunol. 2018 Apr 13;9:754
pubmed: 29706967
Heart. 2015 Apr;101(7):537-45
pubmed: 25605654
J Vis Exp. 2016 Sep 13;(115):
pubmed: 27685659
Pediatr Infect Dis J. 2010 Mar;29(3):288
pubmed: 20190619
Shock. 2017 Dec;48(6):651-656
pubmed: 28682940
Burns. 2017 Mar;43(2):297-303
pubmed: 28341255
Br J Anaesth. 2021 Sep;127(3):365-375
pubmed: 34229833
Immunology. 2020 Jan;159(1):63-74
pubmed: 31573680
J Innate Immun. 2018;10(3):202-214
pubmed: 29455206
Cytometry A. 2017 May;91(5):470-481
pubmed: 28264140
PLoS One. 2012;7(1):e30912
pubmed: 22303471
Integr Biol (Camb). 2013 Mar;5(3):624-30
pubmed: 23328993
ACS Omega. 2021 Nov 12;6(46):31046-31057
pubmed: 34841147
Anesthesiology. 2002 Jul;97(1):215-52
pubmed: 12131125
Stem Cells. 2006 Mar;24(3):793-9
pubmed: 16210405
Blood Transfus. 2017 May;15(3):239-248
pubmed: 28518051
Sci Rep. 2019 Dec 18;9(1):19388
pubmed: 31852983
Cell J. 2013 Fall;15(3):218-23
pubmed: 24027662
Ann Thorac Surg. 2015 Jun;99(6):2061-9
pubmed: 25795297
Neurophotonics. 2014 Oct;1(2):020901
pubmed: 26157976
Histol Histopathol. 2018 May;33(5):417-432
pubmed: 28990642
J Biophotonics. 2013 May;6(5):393-7
pubmed: 22887897
J Lab Physicians. 2019 Jan-Mar;11(1):29-33
pubmed: 30983799
Drugs. 1975;9(1):19-76
pubmed: 1092540
Atherosclerosis. 2005 Dec;183(2):316-21
pubmed: 15996672
Thromb Haemost. 2017 Dec;117(12):2346-2355
pubmed: 29212122
Adv Drug Deliv Rev. 2015 Jul 15;89:121-34
pubmed: 25809988
J Cardiothorac Vasc Anesth. 2009 Apr;23(2):223-31
pubmed: 18930659
Cytokine. 2018 Jul;107:130-136
pubmed: 29402722
Circ J. 2018 Feb 23;82(3):677-683
pubmed: 29238008
Sensors (Basel). 2013 Mar 28;13(4):4170-91
pubmed: 23539026
ASAIO J. 2004 Nov-Dec;50(6):611-8
pubmed: 15672797
PLoS One. 2015 Aug 11;10(8):e0135527
pubmed: 26263001
Appl Opt. 2008 Feb 1;47(4):A52-61
pubmed: 18239699
Cells. 2021 Sep 29;10(10):
pubmed: 34685568
Nat Methods. 2014 Dec;11(12):1221-8
pubmed: 25423019
Assay Drug Dev Technol. 2013 Mar;11(2):101-7
pubmed: 23062077
PLoS One. 2014 Sep 24;9(9):e107317
pubmed: 25251440
J Immunol. 1998 Jun 1;160(11):5294-9
pubmed: 9605127
J Cardiothorac Surg. 2019 Dec 16;14(1):217
pubmed: 31842939
Am J Physiol Cell Physiol. 2008 Aug;295(2):C538-44
pubmed: 18562484
Br J Anaesth. 2017 Oct 1;119(4):637-644
pubmed: 29121297
Cytometry B Clin Cytom. 2016 Jul;90(4):376-86
pubmed: 26130241
Front Cardiovasc Med. 2017 Mar 15;4:12
pubmed: 28361055