In Vivo Evaluation of ECP Peptide Analogues for the Treatment of
AMPs
ECP
Gram-negative bacteria
LPS
infection
murine model
Journal
Biomedicines
ISSN: 2227-9059
Titre abrégé: Biomedicines
Pays: Switzerland
ID NLM: 101691304
Informations de publication
Date de publication:
05 Feb 2022
05 Feb 2022
Historique:
received:
28
12
2021
revised:
31
01
2022
accepted:
02
02
2022
entrez:
25
2
2022
pubmed:
26
2
2022
medline:
26
2
2022
Statut:
epublish
Résumé
Antimicrobial peptides (AMPs) are alternative therapeutics to traditional antibiotics against bacterial resistance. Our previous work identified an antimicrobial region at the N-terminus of the eosinophil cationic protein (ECP). Following structure-based analysis, a 30mer peptide (ECPep-L) was designed that combines antimicrobial action against Gram-negative species with lipopolysaccharides (LPS) binding and endotoxin-neutralization activities. Next, analogues that contain non-natural amino acids were designed to increase serum stability. Here, two analogues were selected for in vivo assays: the all-D version (ECPep-D) and the Arg to Orn version that incorporates a D-amino acid at position 2 (ECPep-2D-Orn). The peptide analogues retained high LPS-binding and anti-endotoxin activities. The peptides efficacy was tested in a murine acute infection model of
Identifiants
pubmed: 35203595
pii: biomedicines10020386
doi: 10.3390/biomedicines10020386
pmc: PMC8962335
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Ministry of Economy, Industry and Competitiveness
ID : PID2019-106123GB-I00
Références
J Microbiol Immunol Infect. 2017 Aug;50(4):405-410
pubmed: 28690026
Antimicrob Agents Chemother. 2002 Jun;46(6):1946-52
pubmed: 12019113
Int J Antimicrob Agents. 2018 Jun;51(6):888-896
pubmed: 29432867
Int J Mol Sci. 2021 Feb 28;22(5):
pubmed: 33671100
J Antimicrob Chemother. 2019 Jan 1;74(1):96-107
pubmed: 30272195
Expert Opin Biol Ther. 2017 Jun;17(6):663-676
pubmed: 28368216
Vaccines (Basel). 2018 Nov 20;6(4):
pubmed: 30463297
Antimicrob Agents Chemother. 2007 Oct;51(10):3471-84
pubmed: 17646423
Lancet Infect Dis. 2020 Sep;20(9):e216-e230
pubmed: 32653070
Nat Genet. 1995 Jun;10(2):219-23
pubmed: 7663519
Nat Protoc. 2008;3(2):163-75
pubmed: 18274517
Microbiol Immunol. 2002;46(11):741-9
pubmed: 12516770
Eur J Med Chem. 2018 May 25;152:590-599
pubmed: 29763807
Front Microbiol. 2019 Jul 25;10:1689
pubmed: 31404242
Amino Acids. 2016 Sep;48(9):2253-60
pubmed: 27270571
Exp Mol Med. 2017 Jun 16;49(6):e345
pubmed: 28620220
Trends Microbiol. 2019 Apr;27(4):323-338
pubmed: 30683453
Int J Antimicrob Agents. 2007 Jun;29(6):630-6
pubmed: 17306965
J Med Chem. 2021 Aug 12;64(15):11472-11482
pubmed: 34342438
Future Med Chem. 2019 Aug;11(16):2047-2050
pubmed: 31538527
Peptides. 2013 Nov;49:131-7
pubmed: 24071034
ChemMedChem. 2015 Oct;10(10):1606-24
pubmed: 26386345
Antimicrob Agents Chemother. 2016 Sep 23;60(10):6313-25
pubmed: 27527084
Curr Pharm Des. 2010;16(28):3185-203
pubmed: 20687878
Bioorg Med Chem. 2013 Jan 1;21(1):205-14
pubmed: 23199484
Sci Rep. 2016 May 13;6:25905
pubmed: 27174567
Front Chem. 2017 Apr 25;5:26
pubmed: 28487853
Protein J. 2013 Aug;32(6):456-66
pubmed: 23925670
Antimicrob Agents Chemother. 2015 May;59(5):2720-5
pubmed: 25712362
Curr Top Med Chem. 2020;20(14):1238-1263
pubmed: 32124698
Arch Microbiol. 2015 May;197(4):521-32
pubmed: 25609230
Front Chem. 2018 Jun 05;6:204
pubmed: 29922648
J Med Chem. 2011 Jul 28;54(14):5237-44
pubmed: 21696142
Molecules. 2019 Aug 02;24(15):
pubmed: 31382389
FEBS Lett. 2016 Nov;590(21):3941-3959
pubmed: 27730639
Antimicrob Agents Chemother. 2015 Nov 16;60(1):592-9
pubmed: 26574005
PLoS One. 2019 Jul 25;14(7):e0220344
pubmed: 31344137
FEMS Microbiol Rev. 2013 Mar;37(2):130-55
pubmed: 22568581
Nat Rev Drug Discov. 2011 Dec 16;11(1):37-51
pubmed: 22173434
Eur J Clin Microbiol Infect Dis. 2010 May;29(5):527-31
pubmed: 20182760
Antimicrob Agents Chemother. 2010 Sep;54(9):3817-24
pubmed: 20606063
Nat Rev Drug Discov. 2020 May;19(5):311-332
pubmed: 32107480
Physiol Genomics. 2013 Oct 1;45(19):877-88
pubmed: 23922129
Eur J Biochem. 2001 Aug;268(15):4233-42
pubmed: 11488917
Innate Immun. 2013 Feb;19(1):86-97
pubmed: 22627784
Front Cell Infect Microbiol. 2016 Dec 27;6:194
pubmed: 28083516
Expert Rev Anti Infect Ther. 2012 Aug;10(8):917-34
pubmed: 23030331
N Engl J Med. 1964 May 14;270:1030-5
pubmed: 14122799
Biomaterials. 2012 Dec;33(34):8841-7
pubmed: 22925814
Proc Natl Acad Sci U S A. 1998 Mar 31;95(7):3708-13
pubmed: 9520431
J Biol Chem. 2004 Jun 18;279(25):26307-13
pubmed: 15096514
Curr Pharm Des. 2002;8(9):795-813
pubmed: 11945172