Phage-Host Interaction Analysis by Flow Cytometry Allows for Rapid and Efficient Screening of Phages.
Pseudomonas aeruginosa
flow cytometry
phages
phage–host interactions
Journal
Antibiotics (Basel, Switzerland)
ISSN: 2079-6382
Titre abrégé: Antibiotics (Basel)
Pays: Switzerland
ID NLM: 101637404
Informations de publication
Date de publication:
27 Jan 2022
27 Jan 2022
Historique:
received:
29
12
2021
revised:
22
01
2022
accepted:
24
01
2022
entrez:
25
2
2022
pubmed:
26
2
2022
medline:
26
2
2022
Statut:
epublish
Résumé
Recently, phages have become popular as an alternative to antibiotics. This increased demand for phage therapy needs rapid and efficient methods to screen phages infecting specific hosts. Existing methods are time-consuming, and for clinical purposes, novel, quick, and reliable screening methods are highly needed. Flow cytometry (FC) allows a quick differentiation and enumeration of bacterial cell populations and has been used to assess in vitro the activity of antimicrobial compounds. In this work, we propose FC as a rapid and reliable method to assess the susceptibility of a bacterial population to phage infection. For that, the interaction of phages vB_PaeM_CEB_DP1 and vB_PaeP_PE3 with
Identifiants
pubmed: 35203767
pii: antibiotics11020164
doi: 10.3390/antibiotics11020164
pmc: PMC8868278
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Fundação para a Ciência e Tecnologia
ID : UIDB/04469/2020 unit
Organisme : Fundação para a Ciência e Tecnologia
ID : PTDC/SAU-PUB/29182/2017 [POCI-01-0145-FEDER-029182]
Organisme : Fundação para a Ciência e Tecnologia
ID : PTDC/BIA-MIC/2312/2020
Organisme : Fundação para a Ciência e Tecnologia
ID : SFRH/BD/143639/2019
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