Lycopene Supplementation to Serum-Free Maturation Medium Improves In Vitro Bovine Embryo Development and Quality and Modulates Embryonic Transcriptomic Profile.
RNA-seq
antioxidant
embryo production
embryo quality
reactive oxygen species
vitrification
Journal
Antioxidants (Basel, Switzerland)
ISSN: 2076-3921
Titre abrégé: Antioxidants (Basel)
Pays: Switzerland
ID NLM: 101668981
Informations de publication
Date de publication:
10 Feb 2022
10 Feb 2022
Historique:
received:
03
01
2022
revised:
02
02
2022
accepted:
08
02
2022
entrez:
25
2
2022
pubmed:
26
2
2022
medline:
26
2
2022
Statut:
epublish
Résumé
Bovine embryos are typically cultured at reduced oxygen tension to lower the impact of oxidative stress on embryo development. However, oocyte in vitro maturation (IVM) is performed at atmospheric oxygen tension since low oxygen during maturation has a negative impact on oocyte developmental competence. Lycopene, a carotenoid, acts as a powerful antioxidant and may protect the oocyte against oxidative stress during maturation at atmospheric oxygen conditions. Here, we assessed the effect of adding 0.2 μM lycopene (antioxidant), 5 μM menadione (pro-oxidant), and their combination on the generation of reactive oxygen species (ROS) in matured oocytes and the subsequent development, quality, and transcriptome of the blastocysts in a bovine in vitro model. ROS fluorescent intensity in matured oocytes was significantly lower in the lycopene group, and the resulting embryos showed a significantly higher blastocyst rate on day 8 and a lower apoptotic cell ratio than all other groups. Transcriptomic analysis disclosed a total of 296 differentially expressed genes (Benjamini-Hochberg-adjusted
Identifiants
pubmed: 35204226
pii: antiox11020344
doi: 10.3390/antiox11020344
pmc: PMC8868338
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : GOA: Ex ovo omnia: from stem cell to oocyte
ID : BOF: GOA 2018000504 (GOA030-18 BOF)
Organisme : GOA: Ex ovo omnia: from stem cell to oocyte
ID : BOF: GOA2018000501
Organisme : Research Foundation - Flanders:
ID : 12Y5220N
Organisme : Research Foundation - Flanders:
ID : 1228821N
Organisme : COLFUTURO and the Ministerio de Ciencia Tecnología Innovación of Colombia:
ID : NA
Références
Proc Biol Sci. 2009 May 22;276(1663):1737-45
pubmed: 19324792
J Assist Reprod Genet. 2013 Dec;30(12):1577-81
pubmed: 24114628
Fertil Steril. 2005 Jul;84(1):228-31
pubmed: 16009190
Genome Biol. 2014;15(12):550
pubmed: 25516281
Theriogenology. 2017 Nov;103:173-184
pubmed: 28800556
Mol Hum Reprod. 2017 Apr 1;23(4):257-268
pubmed: 28130394
BMC Bioinformatics. 2011 Aug 04;12:323
pubmed: 21816040
Gynecol Endocrinol. 2010 Aug;26(8):563-7
pubmed: 20230330
Theriogenology. 2007 Jan 1;67(1):64-72
pubmed: 17049589
Altern Lab Anim. 2002 Mar-Apr;30(2):219-27
pubmed: 11971757
Oxid Med Cell Longev. 2019 Mar 12;2019:1279250
pubmed: 30992736
Biochim Biophys Acta. 2014 Nov;1843(11):2563-2582
pubmed: 24892271
Phytochemistry. 2000 Jun;54(3):233-6
pubmed: 10870177
J Reprod Dev. 2013;59(1):78-84
pubmed: 23154385
Hum Reprod. 2008 Jan;23(1):91-9
pubmed: 17965420
Int J Mol Sci. 2020 Jul 27;21(15):
pubmed: 32727154
J Pineal Res. 2016 Mar;60(2):132-41
pubmed: 26485053
Biol Reprod. 2017 Feb 1;96(2):288-301
pubmed: 28203704
Oncogene. 2006 Oct 30;25(51):6680-4
pubmed: 17072321
Anal Biochem. 2011 Sep 15;416(2):228-30
pubmed: 21684250
BMC Bioinformatics. 2016 Jan 20;17:45
pubmed: 26791995
Biol Reprod. 1996 Jun;54(6):1420-9
pubmed: 8724373
Exp Mol Med. 2012 Feb 29;44(2):69-72
pubmed: 22257881
Hum Reprod. 1986 Feb;1(2):65-8
pubmed: 3558757
Biol Reprod. 1999 Jul;61(1):231-9
pubmed: 10377054
Eur J Pharmacol. 2008 May 31;586(1-3):275-82
pubmed: 18439578
World J Mens Health. 2014 Apr;32(1):1-17
pubmed: 24872947
Reprod Domest Anim. 2017 Aug;52(4):561-569
pubmed: 28295710
Brief Bioinform. 2021 Jan 18;22(1):545-556
pubmed: 32026945
Equine Vet J. 2018 May;50(3):391-397
pubmed: 28833413
Front Pharmacol. 2021 Feb 08;11:629266
pubmed: 33628188
Int J Mol Sci. 2011;12(10):7114-62
pubmed: 22072939
Biom J. 2008 Jun;50(3):346-63
pubmed: 18481363
Int J Mol Sci. 2018 Dec 21;20(1):
pubmed: 30577682
Nat Rev Cancer. 2002 Apr;2(4):301-10
pubmed: 12001991
Theriogenology. 2012 Jul 1;78(1):86-101
pubmed: 22445189
Reprod Biomed Online. 2005 Apr;10(4):497-502
pubmed: 15901458
Nat Rev Mol Cell Biol. 2008 Jan;9(1):47-59
pubmed: 18097445
Toxicol In Vitro. 2004 Feb;18(1):1-12
pubmed: 14630056
Bioinformatics. 2013 Jan 1;29(1):15-21
pubmed: 23104886
Fertil Steril. 2008 Dec;90(6):2080-5
pubmed: 18249400
Theriogenology. 2021 Oct 1;173:48-55
pubmed: 34332201
ALTEX. 2018;35(1):99-118
pubmed: 28800376
Cell Metab. 2012 Oct 3;16(4):420-34
pubmed: 22982021
Fertil Steril. 2004 Apr;81(4):973-6
pubmed: 15066450
BMC Bioinformatics. 2012 Jun 19;13:136
pubmed: 22713124
Free Radic Res. 2011 Aug;45(8):925-40
pubmed: 21615277
Genome Res. 2017 Mar;27(3):491-499
pubmed: 28100584
Pharmacol Rev. 2014 Oct;66(4):1033-79
pubmed: 25244928
BMC Genomics. 2016 Jan 22;17:72
pubmed: 26801242
Reprod Fertil Dev. 2014 Jun;26(5):717-24
pubmed: 23711172
Fertil Steril. 2004 Sep;82(3):593-600
pubmed: 15374701
J Clin Pathol. 2001 Mar;54(3):176-86
pubmed: 11253127
Zygote. 2019 Jun;27(3):180-186
pubmed: 31171044