A fast Myosin super enhancer dictates muscle fiber phenotype through competitive interactions with Myosin genes.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
24 02 2022
24 02 2022
Historique:
received:
26
03
2021
accepted:
04
02
2022
entrez:
25
2
2022
pubmed:
26
2
2022
medline:
13
4
2022
Statut:
epublish
Résumé
The contractile properties of adult myofibers are shaped by their Myosin heavy chain isoform content. Here, we identify by snATAC-seq a 42 kb super-enhancer at the locus regrouping the fast Myosin genes. By 4C-seq we show that active fast Myosin promoters interact with this super-enhancer by DNA looping, leading to the activation of a single promoter per nucleus. A rainbow mouse transgenic model of the locus including the super-enhancer recapitulates the endogenous spatio-temporal expression of adult fast Myosin genes. In situ deletion of the super-enhancer by CRISPR/Cas9 editing demonstrates its major role in the control of associated fast Myosin genes, and deletion of two fast Myosin genes at the locus reveals an active competition of the promoters for the shared super-enhancer. Last, by disrupting the organization of fast Myosin, we uncover positional heterogeneity within limb skeletal muscles that may underlie selective muscle susceptibility to damage in certain myopathies.
Identifiants
pubmed: 35210422
doi: 10.1038/s41467-022-28666-1
pii: 10.1038/s41467-022-28666-1
pmc: PMC8873246
doi:
Substances chimiques
Myosin Heavy Chains
EC 3.6.4.1
Myosins
EC 3.6.4.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1039Informations de copyright
© 2022. The Author(s).
Références
Skelet Muscle. 2015 Jul 15;5:22
pubmed: 26180627
Curr Top Dev Biol. 2012;98:277-301
pubmed: 22305167
Cell. 2017 Mar 23;169(1):13-23
pubmed: 28340338
Semin Pediatr Neurol. 2019 Apr;29:12-22
pubmed: 31060721
Nat Genet. 2016 Aug;48(8):895-903
pubmed: 27376235
Nature. 2018 Feb 8;554(7691):239-243
pubmed: 29420474
Genome Biol. 2016 Jul 05;17(1):148
pubmed: 27380939
Annu Rev Genomics Hum Genet. 2021 Aug 31;22:127-146
pubmed: 33951408
J Clin Invest. 2020 Sep 1;130(9):4710-4725
pubmed: 32544095
Dev Biol. 2011 Nov 15;359(2):303-20
pubmed: 21884692
Graefes Arch Clin Exp Ophthalmol. 2009 Nov;247(11):1505-15
pubmed: 19609551
J Mol Biol. 2020 Feb 7;432(3):643-652
pubmed: 31887284
Cell. 1987 Dec 24;51(6):975-85
pubmed: 3690667
Science. 2018 Jul 27;361(6400):
pubmed: 29930091
Phys Ther. 1993 Dec;73(12):857-67
pubmed: 8248294
Development. 2013 Dec;140(24):4914-25
pubmed: 24301466
J Muscle Res Cell Motil. 2019 Jun;40(2):111-126
pubmed: 31228046
Semin Cell Dev Biol. 2020 Aug;104:51-64
pubmed: 32247726
Nature. 1995 Sep 21;377(6546):209-13
pubmed: 7675106
Nat Rev Genet. 2001 Oct;2(10):769-79
pubmed: 11584293
Annu Rev Neurosci. 2010;33:409-40
pubmed: 20367447
Nat Genet. 2015 Jan;47(1):8-12
pubmed: 25547603
Int J Biochem Cell Biol. 2013 Oct;45(10):2191-9
pubmed: 23702032
Neuromuscul Disord. 2002 May;12(4):343-9
pubmed: 12062251
Elife. 2014 Apr 29;3:e02557
pubmed: 24843030
Elife. 2019 Sep 19;8:
pubmed: 31535973
Skelet Muscle. 2016 Sep 05;6(1):30
pubmed: 27597886
Cell. 2015 May 21;161(5):1012-1025
pubmed: 25959774
Cell Metab. 2013 Feb 5;17(2):162-84
pubmed: 23395166
Genome Res. 2000 Jan;10(1):116-28
pubmed: 10645956
Cell. 2011 Nov 23;147(5):1132-45
pubmed: 22118467
PLoS Genet. 2014 May 22;10(5):e1004386
pubmed: 24852826
Cell. 2016 Jul 14;166(2):358-368
pubmed: 27293191
Cell Rep. 2020 Apr 14;31(2):107503
pubmed: 32294452
Cell. 2013 Nov 7;155(4):934-47
pubmed: 24119843
Mol Cell. 2002 Dec;10(6):1453-65
pubmed: 12504019
EMBO J. 2020 Nov 16;39(22):e105098
pubmed: 32960481
Nat Commun. 2020 Oct 9;11(1):5102
pubmed: 33037211
Blood. 2002 Nov 1;100(9):3077-86
pubmed: 12384402
PLoS Genet. 2017 Jul 27;13(7):e1006903
pubmed: 28749941
J Muscle Res Cell Motil. 2000 May;21(4):345-55
pubmed: 11032345
Mol Cell. 2019 Feb 7;73(3):519-532.e4
pubmed: 30554946
Curr Top Dev Biol. 2014;110:1-73
pubmed: 25248473
Skeletal Radiol. 2019 May;48(5):653-676
pubmed: 30377729
Cell. 2013 Mar 14;152(6):1285-97
pubmed: 23498937
PLoS Biol. 2019 Oct 7;17(10):e3000467
pubmed: 31589602
Eur J Med Genet. 2017 Jun;60(6):312-316
pubmed: 28377322
Nat Genet. 2003 Oct;35(2):190-4
pubmed: 14517543
Cell. 2014 Oct 9;159(2):374-387
pubmed: 25303531
Mol Cell. 1997 Dec;1(1):131-9
pubmed: 9659910
Nat Commun. 2020 Dec 11;11(1):6374
pubmed: 33311464
Genomics. 2001 Apr 1;73(1):56-65
pubmed: 11352566
Acta Neuropathol. 2013 Jan;125(1):3-18
pubmed: 22918376
J Vis Exp. 2017 Nov 6;(129):
pubmed: 29155791
Cell. 2013 Apr 11;153(2):307-19
pubmed: 23582322
Nat Methods. 2017 Oct;14(10):975-978
pubmed: 28825706
Genome Biol. 2008;9(9):R137
pubmed: 18798982
Nat Methods. 2012 Mar 04;9(4):357-9
pubmed: 22388286
Biol Cell. 2003 Sep;95(6):399-406
pubmed: 14519557
Proc Natl Acad Sci U S A. 2020 Nov 24;117(47):29691-29701
pubmed: 33148801
PLoS One. 2016 Apr 26;11(4):e0147669
pubmed: 27115354
Methods Mol Biol. 2016;1480:223-41
pubmed: 27659989
PLoS One. 2014 Jan 27;9(1):e85879
pubmed: 24475057
Cell. 2020 Oct 1;183(1):28-45
pubmed: 32976797
Cell Rep. 2017 Jun 13;19(11):2396-2409
pubmed: 28614723
Wiley Interdiscip Rev Syst Biol Med. 2012 Sep-Oct;4(5):457-73
pubmed: 22811254
Skelet Muscle. 2011 Feb 01;1(1):5
pubmed: 21798083
Dev Biol. 1999 Dec 1;216(1):312-26
pubmed: 10588881
Physiol Rev. 2011 Oct;91(4):1447-531
pubmed: 22013216
Anat Embryol (Berl). 1990;181(6):513-22
pubmed: 2144412
Nat Commun. 2019 Nov 27;10(1):5412
pubmed: 31776347
Nat Commun. 2021 Jan 19;12(1):439
pubmed: 33469032
Cell. 2019 Jun 13;177(7):1888-1902.e21
pubmed: 31178118
Ann Neurol. 2010 Feb;67(2):201-8
pubmed: 20225280
PLoS Genet. 2006 Sep 29;2(9):e156
pubmed: 17009875
Nature. 2012 Apr 11;485(7398):376-80
pubmed: 22495300
J Biol Chem. 2005 Apr 29;280(17):17126-34
pubmed: 15728583
Handb Clin Neurol. 2018;148:549-564
pubmed: 29478600
Biol Rev Camb Philos Soc. 2011 Aug;86(3):564-600
pubmed: 21040371
Genes Dev. 2008 Jan 15;22(2):265-76
pubmed: 18198342
Intern Med. 2021 Jun 15;60(12):1935-1939
pubmed: 33456041
Adv Genet. 2008;61:107-42
pubmed: 18282504
J Appl Physiol (1985). 2003 Mar;94(3):1230-41
pubmed: 12571144
Microsc Res Tech. 2000 Sep 15;50(6):492-9
pubmed: 10998638
Curr Opin Clin Nutr Metab Care. 2013 May;16(3):243-50
pubmed: 23493017
Cell. 2017 Oct 19;171(3):557-572.e24
pubmed: 29053968
Nat Commun. 2020 Dec 11;11(1):6375
pubmed: 33311457
J Exp Biol. 2019 Nov 29;222(Pt 23):
pubmed: 31784473
IUBMB Life. 2008 Dec;60(12):824-33
pubmed: 18767169
Nat Genet. 2016 Dec 28;49(1):2-3
pubmed: 28029159
Development. 2020 Feb 3;147(3):
pubmed: 32014867
BMC Biol. 2017 Jun 9;15(1):48
pubmed: 28599653