Engineered Zinc Finger Protein Targeting 2LTR Inhibits HIV Integration in Hematopoietic Stem and Progenitor Cell-Derived Macrophages: In Vitro Study.


Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
19 Feb 2022
Historique:
received: 12 01 2022
revised: 15 02 2022
accepted: 16 02 2022
entrez: 26 2 2022
pubmed: 27 2 2022
medline: 23 3 2022
Statut: epublish

Résumé

Human hematopoietic stem/progenitor cell (HSPC)-based gene therapy is a promising direction for curing HIV-1-infected individuals. The zinc finger protein (2LTRZFP) designed to target the 2-LTR-circle junction of HIV-1 cDNA was previously reported as an intracellular antiviral molecular scaffold that prevents HIV integration. Here, we elucidate the efficacy and safety of using 2LTRZFP in human CD34

Identifiants

pubmed: 35216446
pii: ijms23042331
doi: 10.3390/ijms23042331
pmc: PMC8875109
pii:
doi:

Substances chimiques

Antigens, CD34 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : the Distinguished Research Professor Grant of the National Research Council of Thailand
ID : NRCT 808/2563
Organisme : the Office of National Higher Education Science Research and Innovation Policy Council (NXPO), Thailand, through Program Management Unit for Competitiveness (PMU C)
ID : C10F630145
Organisme : the Program Management Unit for Human Resources and Institutional Development, Research and Innovation
ID : B05F630102
Organisme : the Permanent Secretary, Ministry of Higher Education, Science, Research and Innovation
ID : RGNS 63-067
Organisme : Fundamental Fund 2022, Chiang Mai University
Organisme : CMU Junior Research Fellowship Program
Organisme : AMS Research Grant of Faculty of Associated Medical Sciences, Chiang Mai University
Organisme : King Mongkut's Institute of Technology Ladkrabang
ID : 2563-0216006

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Auteurs

Koollawat Chupradit (K)

Siriraj Center for Regenerative Medicine, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
Center of Biomolecular Therapy and Diagnostic, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.

Wannisa Khamaikawin (W)

Faculty of Medicine, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.

Supachai Sakkhachornphop (S)

Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.

Chaniporn Puaninta (C)

Department of Anatomy, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.

Bruce E Torbett (BE)

Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA.

Suparerk Borwornpinyo (S)

Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
Excellent Center for Drug Discovery, Mahidol University, Bangkok 10400, Thailand.

Suradej Hongeng (S)

Excellent Center for Drug Discovery, Mahidol University, Bangkok 10400, Thailand.
Department of Pediatrics, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand.

Methichit Wattanapanitch (M)

Siriraj Center for Regenerative Medicine, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.

Chatchai Tayapiwatana (C)

Center of Biomolecular Therapy and Diagnostic, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
Division of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
Center of Innovative Immunodiagnostic Development, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.

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