SARS-CoV-2 spike antigen quantification by targeted mass spectrometry of a virus-based vaccine.
Mass spectrometry
Quantification
SARS-CoV-2
Spike
Vaccine
Journal
Journal of virological methods
ISSN: 1879-0984
Titre abrégé: J Virol Methods
Pays: Netherlands
ID NLM: 8005839
Informations de publication
Date de publication:
05 2022
05 2022
Historique:
received:
22
11
2021
revised:
17
02
2022
accepted:
18
02
2022
pubmed:
27
2
2022
medline:
16
3
2022
entrez:
26
2
2022
Statut:
ppublish
Résumé
The spike glycoprotein mediates virus binding to the host cells and is a key target for vaccines development. One SARS-CoV-2 vaccine is based on vesicular stomatitis virus (VSV), in which the native surface glycoprotein has been replaced by the SARS-CoV-2 spike protein (VSV-ΔG-spike). The titer of the virus is quantified by the plaque forming unit (PFU) assay, but there is no method for spike protein quantitation as an antigen in a VSV-based vaccine. Here, we describe a mass spectrometric (MS) spike protein quantification method, applied to VSV-ΔG-spike based vaccine. Proof of concept of this method, combining two different sample preparations, is shown for complex matrix samples, produced during the vaccine manufacturing processes. Total spike levels were correlated with results from activity assays, and ranged between 0.3-0.5 μg of spike protein per 10
Identifiants
pubmed: 35217103
pii: S0166-0934(22)00045-3
doi: 10.1016/j.jviromet.2022.114498
pmc: PMC8863330
pii:
doi:
Substances chimiques
COVID-19 Vaccines
0
Spike Glycoprotein, Coronavirus
0
Viral Vaccines
0
spike protein, SARS-CoV-2
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
114498Informations de copyright
Copyright © 2022 Elsevier B.V. All rights reserved.
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