IBD-associated G protein-coupled receptor 65 variant compromises signalling and impairs key functions involved in inflammation.

HEK293 Cells Humans Inflammasomes / metabolism Inflammation / metabolism Inflammatory Bowel Diseases / genetics Interleukin-1beta NLR Family, Pyrin Domain-Containing 3 Protein Receptors, G-Protein-Coupled / genetics
Actin remodeling Bacterial phagocytosis G proteins GPR65 NLRP3 inflammasome

Journal

Cellular signalling
ISSN: 1873-3913
Titre abrégé: Cell Signal
Pays: England
ID NLM: 8904683

Informations de publication

Date de publication:
05 2022
Historique:
received: 23 11 2021
revised: 06 02 2022
accepted: 21 02 2022
pubmed: 27 2 2022
medline: 26 4 2022
entrez: 26 2 2022
Statut: ppublish

Résumé

Inflammatory bowel diseases (IBD) result in chronic inflammation of the gastrointestinal tract. Genetic studies have shown that the GPR65 gene, as well as its missense coding variant, GPR65*Ile231Leu, is associated with IBD. We aimed to define the signalling and biological pathways downstream of GPR65 activation and evaluate the impact of GPR65*231Leu on these. We used HEK 293 cells stably expressing GPR65 and deficient for either Gα We confirmed that GPR65 signals via the Gα We demonstrate that GPR65 exerts its effects through Gα

Sections du résumé

BACKGROUND AND AIMS
Inflammatory bowel diseases (IBD) result in chronic inflammation of the gastrointestinal tract. Genetic studies have shown that the GPR65 gene, as well as its missense coding variant, GPR65*Ile231Leu, is associated with IBD. We aimed to define the signalling and biological pathways downstream of GPR65 activation and evaluate the impact of GPR65*231Leu on these.
METHODS
We used HEK 293 cells stably expressing GPR65 and deficient for either Gα
RESULTS
We confirmed that GPR65 signals via the Gα
CONCLUSIONS
We demonstrate that GPR65 exerts its effects through Gα

Identifiants

DOI: 10.1016/j.cellsig.2022.110294 PMID: 35218908 PMC: PMC9536022
pubmed: 35218908
pii: S0898-6568(22)00054-7
doi: 10.1016/j.cellsig.2022.110294
pmc: PMC9536022
mid: NIHMS1836470
pii:
doi:

Substances chimiques

GPR65 protein, human 0
Inflammasomes 0
Interleukin-1beta 0
NLR Family, Pyrin Domain-Containing 3 Protein 0
Receptors, G-Protein-Coupled 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

110294

Subventions

Organisme : NIDDK NIH HHS
ID : R01 DK064869
Pays : United States
Organisme : NIDDK NIH HHS
ID : U01 DK062432
Pays : United States
Organisme : CIHR
ID : PJT-156298
Pays : Canada

Informations de copyright

Copyright © 2022 Elsevier Inc. All rights reserved.

Références

J Crohns Colitis. 2018 Feb 28;12(3):355-368
pubmed: 29136128
Oncogene. 2016 Jul 21;35(29):3771-80
pubmed: 26616858
Nat Genet. 2015 Sep;47(9):979-986
pubmed: 26192919
Cell Mol Life Sci. 2011 Dec;68(23):3823-41
pubmed: 21744067
Nat Genet. 2011 Oct 09;43(11):1066-73
pubmed: 21983784
Small GTPases. 2015;6(2):89-99
pubmed: 25941749
Cells. 2020 Jan 06;9(1):
pubmed: 31935860
J Biol Chem. 2004 Oct 29;279(44):45626-33
pubmed: 15326175
Inflamm Bowel Dis. 2012 Jun;18(6):1072-80
pubmed: 21994190
J Immunol. 2021 Jul 1;207(1):101-109
pubmed: 34135065
Nat Rev Immunol. 2019 Aug;19(8):477-489
pubmed: 31036962
Immunity. 2016 Jun 21;44(6):1392-405
pubmed: 27287411
J Biol Chem. 2008 Aug 22;283(34):23429-39
pubmed: 18541530
Immunol Cell Biol. 2018 Feb;96(2):128-136
pubmed: 29363187
Front Immunol. 2019 Feb 28;10:276
pubmed: 30873162
Nature. 2017 Jul 13;547(7662):173-178
pubmed: 28658209
J Crohns Colitis. 2019 Feb 01;13(2):245-258
pubmed: 30535144
J Immunol. 2009 Mar 1;182(5):3243-51
pubmed: 19234222
Nature. 2012 Nov 1;491(7422):119-24
pubmed: 23128233
Cells. 2019 Jul 17;8(7):
pubmed: 31319592
Signal Transduct Target Ther. 2021 Jan 8;6(1):7
pubmed: 33414387
World J Gastroenterol. 2014 Mar 14;20(10):2664-72
pubmed: 24627602
J Neurochem. 2014 May;129(4):683-95
pubmed: 24447140
Trends Pharmacol Sci. 2018 May;39(5):481-493
pubmed: 29548548
Front Immunol. 2020 Mar 18;11:410
pubmed: 32256490
Sci Transl Med. 2019 Sep 25;11(511):
pubmed: 31554740
Nat Rev Immunol. 2014 May;14(5):329-42
pubmed: 24751956
Sci Rep. 2017 Feb 01;7:41415
pubmed: 28145512
Inflamm Bowel Dis. 2015 Jun;21(6):1269-81
pubmed: 25856770
J Biol Chem. 2013 May 10;288(19):13410-9
pubmed: 23530046
Biochim Biophys Acta Mol Basis Dis. 2022 Jan 1;1868(1):166288
pubmed: 34628032
J Crohns Colitis. 2017 Jun 01;11(6):737-750
pubmed: 27993998
Innate Immun. 2021 Feb;27(2):133-142
pubmed: 33241977
BMC Gastroenterol. 2019 Jan 7;19(1):2
pubmed: 30616622
J Immunol. 2016 Aug 15;197(4):1425-34
pubmed: 27402702
Sci Signal. 2017 Jun 20;10(484):
pubmed: 28634209
J Biol Chem. 2005 Mar 11;280(10):9083-7
pubmed: 15618224
Science. 2018 Mar 9;359(6380):1161-1166
pubmed: 29420262
Nat Genet. 2007 May;39(5):596-604
pubmed: 17435756
World J Gastroenterol. 2020 Mar 28;26(12):1242-1261
pubmed: 32256014
Int J Mol Sci. 2015 Dec 04;16(12):28931-42
pubmed: 26690120
PLoS One. 2020 May 29;15(5):e0233543
pubmed: 32469933
Nat Rev Drug Discov. 2013 Jan;12(1):25-34
pubmed: 23237917
Intest Res. 2018 Jan;16(1):26-42
pubmed: 29422795
Nat Rev Immunol. 2015 Sep 15;15(9):559-73
pubmed: 26292640
Nature. 2015 Nov 26;527(7579):477-83
pubmed: 26550826
Sci Rep. 2017 Aug 21;7(1):8870
pubmed: 28827659
J Biochem. 2011 Oct;150(4):357-69
pubmed: 21873336
Nat Rev Gastroenterol Hepatol. 2019 Sep;16(9):531-543
pubmed: 31312042
Proc Natl Acad Sci U S A. 2005 Feb 1;102(5):1632-7
pubmed: 15665078
Nat Genet. 2010 Dec;42(12):1118-25
pubmed: 21102463
Front Immunol. 2016 Mar 31;7:123
pubmed: 27065076
Sci Rep. 2020 May 29;10(1):8779
pubmed: 32471984
Sci Rep. 2017 Oct 23;7(1):13741
pubmed: 29061999

Auteurs

Virginie Mercier (V)

Research Center, Montreal Heart Institute, 5000 rue Bélanger, Montreal, Quebec H1T 1C8, Canada.

Gabrielle Boucher (G)

Research Center, Montreal Heart Institute, 5000 rue Bélanger, Montreal, Quebec H1T 1C8, Canada.

Dominic Devost (D)

Department of Pharmacology and Therapeutics, McIntyre Medical Building, 3655 Prom. Sir William Osler, Room 1303, McGill University, Montreal, Quebec H3G 1Y6, Canada.

Kyla Bourque (K)

Department of Pharmacology and Therapeutics, McIntyre Medical Building, 3655 Prom. Sir William Osler, Room 1303, McGill University, Montreal, Quebec H3G 1Y6, Canada.

Azadeh Alikashani (A)

Research Center, Montreal Heart Institute, 5000 rue Bélanger, Montreal, Quebec H1T 1C8, Canada.

Claudine Beauchamp (C)

Research Center, Montreal Heart Institute, 5000 rue Bélanger, Montreal, Quebec H1T 1C8, Canada.

Alain Bitton (A)

Division of Gastroenterology, McGill University Health Centre, Montreal, Quebec H3A 0G4, Canada.

Sylvain Foisy (S)

Research Center, Montreal Heart Institute, 5000 rue Bélanger, Montreal, Quebec H1T 1C8, Canada; Faculty of Medicine, Université de Montréal, C.P. 6128, succursale Centre-ville, Montreal, Quebec H3C 3J7, Canada.

Philippe Goyette (P)

Research Center, Montreal Heart Institute, 5000 rue Bélanger, Montreal, Quebec H1T 1C8, Canada.

Guy Charron (G)

Research Center, Montreal Heart Institute, 5000 rue Bélanger, Montreal, Quebec H1T 1C8, Canada.

Terence E Hébert (TE)

Department of Pharmacology and Therapeutics, McIntyre Medical Building, 3655 Prom. Sir William Osler, Room 1303, McGill University, Montreal, Quebec H3G 1Y6, Canada. Electronic address: terence.hebert@mcgill.ca.

John D Rioux (JD)

Research Center, Montreal Heart Institute, 5000 rue Bélanger, Montreal, Quebec H1T 1C8, Canada; Faculty of Medicine, Université de Montréal, C.P. 6128, succursale Centre-ville, Montreal, Quebec H3C 3J7, Canada. Electronic address: john.david.rioux@umontreal.ca.

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female

Evaluation of Low-Value Services Across Major Medicare Advantage Insurers and Traditional Medicare.

Ciara Duggan, Adam L Beckman, Ishani Ganguli et al.
1.00
Humans United States Aged Cross-Sectional Studies Medicare Part C

Effectiveness of Virtual Yoga for Chronic Low Back Pain: A Randomized Clinical Trial.

Hallie Tankha, Devyn Gaskins, Amanda Shallcross et al.
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH

questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules HEK293 › IBD-associated G protein-coupled receptor 65...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › IBD-associated G protein-coupled receptor 65...
questionsmedicales.fr › Structures macromoléculaires › Complexes multiprotéiques › Inflammasomes › IBD-associated G protein-coupled receptor 65...
questionsmedicales.fr › États, signes et symptômes pathologiques › Processus pathologiques › Inflammation › IBD-associated G protein-coupled receptor 65...
questionsmedicales.fr › Maladies de l'appareil digestif › Maladies gastro-intestinales › Maladies intestinales › Maladies inflammatoires intestinales › IBD-associated G protein-coupled receptor 65...
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Cytokines › Monokines › Interleukine-1 › Interleukine-1 bêta › IBD-associated G protein-coupled receptor 65...
questionsmedicales.fr › Acides aminés, peptides et protéines › Peptides › Protéines NLR › Protéine-3 de la famille des NLR contenant un domaine pyrine › IBD-associated G protein-coupled receptor 65...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Récepteurs couplés aux protéines G › IBD-associated G protein-coupled receptor 65...