Telomere length in leucocytes and solid tissues of young and aged rats.
Sprague Dawley rats
aging
shelterin
telomerase
telomeres
Journal
Aging
ISSN: 1945-4589
Titre abrégé: Aging (Albany NY)
Pays: United States
ID NLM: 101508617
Informations de publication
Date de publication:
27 02 2022
27 02 2022
Historique:
received:
18
12
2021
accepted:
22
02
2022
pubmed:
28
2
2022
medline:
26
4
2022
entrez:
27
2
2022
Statut:
ppublish
Résumé
Telomeres are protective nucleoprotein structures at the end of chromosomes that shorten with age. Telomere length (TL) in peripheral blood mononuclear cells (PBMCs) has been proposed as surrogate marker for TL in the entire organism. Solid evidence that supports this concept is lacking. Relative TL (RTL) was measured in PBMCS and multiple solid tissues from 24 young (4 months) and 24 aged (14 months) Sprague-Dawley (SD) rats. The mRNA expression of telomerase (TERT) and shelterin proteins TERF-1 and TERF-2 was also measured. Mean RTL in PBMCs and solid tissues of young rats ranged from 0.64 ± 0.26 in large intestine to 1.07 ± 0.22 in skeletal muscle. RTL in PBMCs correlated with that in kidney (r = 0.315, p = 0.008), skeletal muscle (r = 0.276, p = 0.022), liver (r = 0.269, p = 0.033), large intestine (r = -0.463, p = 7.035E-5) and aorta (r = -0.273, p = 0.028). A significant difference of RTL between young and aged animals was only observed in aorta (0.98 ± 0.15 vs. 0.76 ± 0.11, p = 1.987E-6), lung (0.76 ± 0.14 vs. 0.85 ± 0.14, p = 0.024) and visceral fat (0.83 ± 0.14 vs. 0.92 ± 0.15, p = 0.44). The expression of TERT significantly differed between the tested organs with highest levels in liver and kidney. Age-related differences in TERT expression were found in PBMCs, skeletal muscle, and visceral fat. mRNA expression of TERF-1 and TERF-2 was tissue-specific with the highest levels in liver. Age-related differences in TERF-1 and TERF-2 expression were inconsistent. The present study questions the utility of RTL in PBMCs as a biomarker for the individual assessment of aging.
Sections du résumé
BACKGROUND
Telomeres are protective nucleoprotein structures at the end of chromosomes that shorten with age. Telomere length (TL) in peripheral blood mononuclear cells (PBMCs) has been proposed as surrogate marker for TL in the entire organism. Solid evidence that supports this concept is lacking.
METHODS
Relative TL (RTL) was measured in PBMCS and multiple solid tissues from 24 young (4 months) and 24 aged (14 months) Sprague-Dawley (SD) rats. The mRNA expression of telomerase (TERT) and shelterin proteins TERF-1 and TERF-2 was also measured.
RESULTS
Mean RTL in PBMCs and solid tissues of young rats ranged from 0.64 ± 0.26 in large intestine to 1.07 ± 0.22 in skeletal muscle. RTL in PBMCs correlated with that in kidney (r = 0.315, p = 0.008), skeletal muscle (r = 0.276, p = 0.022), liver (r = 0.269, p = 0.033), large intestine (r = -0.463, p = 7.035E-5) and aorta (r = -0.273, p = 0.028). A significant difference of RTL between young and aged animals was only observed in aorta (0.98 ± 0.15 vs. 0.76 ± 0.11, p = 1.987E-6), lung (0.76 ± 0.14 vs. 0.85 ± 0.14, p = 0.024) and visceral fat (0.83 ± 0.14 vs. 0.92 ± 0.15, p = 0.44). The expression of TERT significantly differed between the tested organs with highest levels in liver and kidney. Age-related differences in TERT expression were found in PBMCs, skeletal muscle, and visceral fat. mRNA expression of TERF-1 and TERF-2 was tissue-specific with the highest levels in liver. Age-related differences in TERF-1 and TERF-2 expression were inconsistent.
CONCLUSIONS
The present study questions the utility of RTL in PBMCs as a biomarker for the individual assessment of aging.
Identifiants
pubmed: 35220278
pii: 203922
doi: 10.18632/aging.203922
pmc: PMC8908913
doi:
Substances chimiques
RNA, Messenger
0
Telomerase
EC 2.7.7.49
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1713-1728Références
Genes Dev. 2014 Nov 15;28(22):2464-76
pubmed: 25403178
Circulation. 2009 Dec 15;120(24):2438-47
pubmed: 19948976
Am J Epidemiol. 2009 Feb 1;169(3):323-9
pubmed: 19056834
J Gerontol A Biol Sci Med Sci. 2012 Sep;67(9):911-26
pubmed: 22389464
Am J Hum Biol. 2011 Jan-Feb;23(1):100-6
pubmed: 21080476
Oncotarget. 2017 Jul 4;8(27):45008-45019
pubmed: 28410238
Biomedicines. 2021 Sep 27;9(10):
pubmed: 34680452
Biomed Res Int. 2013;2013:601368
pubmed: 24455708
J Biol Chem. 2018 Apr 13;293(15):5731-5745
pubmed: 29440390
Science. 2020 Sep 11;369(6509):
pubmed: 32913074
Physiol Res. 2014;63(Suppl 3):S343-50
pubmed: 25428739
Proc Natl Acad Sci U S A. 1995 May 23;92(11):4818-22
pubmed: 7761406
J Gerontol A Biol Sci Med Sci. 2011 Mar;66(3):312-9
pubmed: 21310811
Geroscience. 2021 Apr;43(2):645-654
pubmed: 33469834
Obes Res Clin Pract. 2017 Jan - Feb;11(1):51-62
pubmed: 26895795
Eur J Nutr. 2017 Aug;56(5):1887-1898
pubmed: 27379829
Aging Cell. 2013 Aug;12(4):615-21
pubmed: 23601089
Int J Obes (Lond). 2012 Sep;36(9):1176-9
pubmed: 22005719
EMBO J. 2001 Oct 1;20(19):5532-40
pubmed: 11574485
Atherosclerosis. 2008 Oct;200(2):271-7
pubmed: 18280483
Geriatrics (Basel). 2019 Feb 23;4(1):
pubmed: 31023993
Clin Chem Lab Med. 2015 Oct;53(11):1661-78
pubmed: 25803084
Exp Physiol. 2017 Apr 1;102(4):397-410
pubmed: 28166612
Mech Ageing Dev. 2012 Jun;133(6):373-7
pubmed: 22561920
Ageing Res Rev. 2013 Mar;12(2):509-19
pubmed: 23333817
Science. 2001 May 11;292(5519):1075-6
pubmed: 11352055
Nucleic Acids Res. 2002 May 15;30(10):e47
pubmed: 12000852
J Am Coll Cardiol. 2008 Aug 5;52(6):470-82
pubmed: 18672169
Curr Opin Cell Biol. 2018 Jun;52:1-7
pubmed: 29253739
Int J Epidemiol. 2009 Dec;38(6):1725-34
pubmed: 19666704
Aging (Albany NY). 2020 Jan 3;12(1):359-369
pubmed: 31901896
Science. 2001 Jun 15;292(5524):2075-7
pubmed: 11408657
Genes Dev. 2005 Sep 15;19(18):2100-10
pubmed: 16166375
Cell Rep. 2021 May 18;35(7):109154
pubmed: 34010660
Philos Trans R Soc Lond B Biol Sci. 2018 Mar 5;373(1741):
pubmed: 29335378
Clin Chem Lab Med. 2018 Jul 26;56(8):1210-1222
pubmed: 29494336
Ageing Res Rev. 2018 Dec;48:11-20
pubmed: 30254001
Nucleic Acids Res. 2003 Mar 1;31(5):1576-83
pubmed: 12595567
J Biol Chem. 2020 Jul 24;295(30):10245-10254
pubmed: 32444498
Mol Carcinog. 1999 Mar;24(3):209-17
pubmed: 10204805
Brain Behav Immun. 2013 Feb;28:16-24
pubmed: 23010452
Proc Natl Acad Sci U S A. 1989 Sep;86(18):7049-53
pubmed: 2780561
Proc Natl Acad Sci U S A. 2004 Dec 7;101(49):17312-5
pubmed: 15574496
BMC Res Notes. 2012 Dec 27;5:697
pubmed: 23270390
J Nutr Biochem. 2011 Oct;22(10):895-901
pubmed: 21429730
Aging (Albany NY). 2016 Jul;8(7):1398-415
pubmed: 27391763
Mutat Res. 2012 Feb 1;730(1-2):59-67
pubmed: 21663926
CMAJ. 1994 Feb 15;150(4):489-95
pubmed: 8313261