Challenges to, and prospects for, reverse engineering the gastrointestinal tract using organoids.

co-culture gastrointestinal tract multiregion organoids organoid fusion organoid-on-a-chip organoids

Journal

Trends in biotechnology
ISSN: 1879-3096
Titre abrégé: Trends Biotechnol
Pays: England
ID NLM: 8310903

Informations de publication

Date de publication:
08 2022
Historique:
received: 19 10 2021
revised: 09 01 2022
accepted: 10 01 2022
pubmed: 1 3 2022
medline: 19 7 2022
entrez: 28 2 2022
Statut: ppublish

Résumé

For over a decade, organoids mimicking the development, physiology, and disease of the digestive system have been a topic of broad interest and intense study. Establishing organoid models that recapitulate all distinct regions of the gastrointestinal tract (GIT) has proven challenging since each tissue surrogate requires tailor-made modifications of the original protocol to generate intestinal organoids. In this review, we discuss the challenges and current advances of the GIT organoid models. Moreover, we envision the next-generation GIT organoids as integrated organoid models, able to recapitulate structural and functional characteristics of multiple regions of the digestive tube in a single in vitro model. We discuss these new trends and provide an outlook for the future of GIT in vitro models.

Identifiants

pubmed: 35221125
pii: S0167-7799(22)00006-3
doi: 10.1016/j.tibtech.2022.01.006
pii:
doi:

Types de publication

Journal Article Review Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

932-944

Informations de copyright

Copyright © 2022 The Author(s). Published by Elsevier Ltd.. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

Auteurs

Panagiota Kakni (P)

Department of Instructive Biomaterials Engineering, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Universiteitssingel 40, 6229, ER, Maastricht, The Netherlands.

Roman Truckenmüller (R)

Department of Instructive Biomaterials Engineering, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Universiteitssingel 40, 6229, ER, Maastricht, The Netherlands.

Pamela Habibović (P)

Department of Instructive Biomaterials Engineering, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Universiteitssingel 40, 6229, ER, Maastricht, The Netherlands.

Stefan Giselbrecht (S)

Department of Instructive Biomaterials Engineering, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Universiteitssingel 40, 6229, ER, Maastricht, The Netherlands. Electronic address: s.giselbrecht@maastrichtuniversity.nl.

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Classifications MeSH