Subcellular localization of hippocampal ryanodine receptor 2 and its role in neuronal excitability and memory.

Animals Hippocampus / metabolism Mice Neurons / metabolism Presynaptic Terminals / metabolism Pyramidal Cells / metabolism Ryanodine Receptor Calcium Release Channel / genetics

Journal

Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179

Informations de publication

Date de publication:
01 03 2022
Historique:
received: 24 08 2021
accepted: 01 02 2022
entrez: 2 3 2022
pubmed: 3 3 2022
medline: 5 4 2022
Statut: epublish

Résumé

Ryanodine receptor 2 (RyR2) is abundantly expressed in the heart and brain. Mutations in RyR2 are associated with both cardiac arrhythmias and intellectual disability. While the mechanisms of RyR2-linked arrhythmias are well characterized, little is known about the mechanism underlying RyR2-associated intellectual disability. Here, we employed a mouse model expressing a green fluorescent protein (GFP)-tagged RyR2 and a specific GFP probe to determine the subcellular localization of RyR2 in hippocampus. GFP-RyR2 was predominantly detected in the soma and dendrites, but not the dendritic spines of CA1 pyramidal neurons or dentate gyrus granular neurons. GFP-RyR2 was also detected within the mossy fibers in the stratum lucidum of CA3, but not in the presynaptic terminals of CA1 neurons. An arrhythmogenic RyR2-R4496C

Identifiants

DOI: 10.1038/s42003-022-03124-2 PMID: 35233070 PMC: PMC8888588
pubmed: 35233070
doi: 10.1038/s42003-022-03124-2
pii: 10.1038/s42003-022-03124-2
pmc: PMC8888588
doi:

Substances chimiques

Ryanodine Receptor Calcium Release Channel 0
ryanodine receptor 2. mouse 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

183

Subventions

Organisme : CIHR
Pays : Canada

Informations de copyright

© 2022. The Author(s).

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Auteurs

Florian Hiess (F)

Libin Cardiovascular Institute, Department of Physiology and Pharmacology, University of Calgary, Calgary, AB, T2N 4N1, Canada.

Jinjing Yao (J)

Libin Cardiovascular Institute, Department of Physiology and Pharmacology, University of Calgary, Calgary, AB, T2N 4N1, Canada.
ORCID: 0000-0002-4602-7159

Zhenpeng Song (Z)

Libin Cardiovascular Institute, Department of Physiology and Pharmacology, University of Calgary, Calgary, AB, T2N 4N1, Canada.

Bo Sun (B)

Libin Cardiovascular Institute, Department of Physiology and Pharmacology, University of Calgary, Calgary, AB, T2N 4N1, Canada.

Zizhen Zhang (Z)

Hotchkiss Brain Institute, Department of Physiology and Pharmacology, University of Calgary, Calgary, AB, Canada.

Junting Huang (J)

Hotchkiss Brain Institute, Department of Physiology and Pharmacology, University of Calgary, Calgary, AB, Canada.

Lina Chen (L)

Hotchkiss Brain Institute, Department of Physiology and Pharmacology, University of Calgary, Calgary, AB, Canada.

Adam Institoris (A)

Hotchkiss Brain Institute, Department of Physiology and Pharmacology, University of Calgary, Calgary, AB, Canada.

John Paul Estillore (JP)

Libin Cardiovascular Institute, Department of Physiology and Pharmacology, University of Calgary, Calgary, AB, T2N 4N1, Canada.

Ruiwu Wang (R)

Libin Cardiovascular Institute, Department of Physiology and Pharmacology, University of Calgary, Calgary, AB, T2N 4N1, Canada.

Henk E D J Ter Keurs (HEDJ)

Libin Cardiovascular Institute, Department of Cardiovascular Science, Department of Medicine, University of Calgary, Calgary, AB, Canada.

Peter K Stys (PK)

Hotchkiss Brain Institute, Department of Physiology and Pharmacology, University of Calgary, Calgary, AB, Canada.
Department of Clinical Neurosciences, University of Calgary, Calgary, AB, Canada.
ORCID: 0000-0003-0326-4194

Grant R Gordon (GR)

Hotchkiss Brain Institute, Department of Physiology and Pharmacology, University of Calgary, Calgary, AB, Canada.

Gerald W Zamponi (GW)

Hotchkiss Brain Institute, Department of Physiology and Pharmacology, University of Calgary, Calgary, AB, Canada.

Anutosh Ganguly (A)

Department of Microbiology, Immunology, and Infectious Diseases, University of Calgary, Calgary, AB, Canada.

S R Wayne Chen (SRW)

Libin Cardiovascular Institute, Department of Physiology and Pharmacology, University of Calgary, Calgary, AB, T2N 4N1, Canada. swchen@ucalgary.ca.
Hotchkiss Brain Institute, Department of Physiology and Pharmacology, University of Calgary, Calgary, AB, Canada. swchen@ucalgary.ca.
ORCID: 0000-0001-5808-0304

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questionsmedicales.fr › Cellules › Neurones › Subcellular localization of hippocampal...
questionsmedicales.fr › Cellules › Neurones › Neurofibres › Axones › Terminaisons présynaptiques › Subcellular localization of hippocampal...
questionsmedicales.fr › Cellules › Neurones › Cellules pyramidales › Subcellular localization of hippocampal...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Canaux ioniques › Canaux calciques › Canal de libération du calcium du récepteur à la ryanodine › Subcellular localization of hippocampal...