Immunization with synthetic SARS-CoV-2 S glycoprotein virus-like particles protects macaques from infection.
Animals
Antibodies, Neutralizing
/ immunology
Antibodies, Viral
/ immunology
COVID-19
/ epidemiology
COVID-19 Vaccines
/ administration & dosage
Chlorocebus aethiops
Disease Models, Animal
HEK293 Cells
Humans
Immunoglobulin A
/ immunology
Immunoglobulin G
/ immunology
Liposomes
Macaca fascicularis
Male
Pandemics
/ prevention & control
SARS-CoV-2
/ immunology
Spike Glycoprotein, Coronavirus
/ immunology
Th1 Cells
/ immunology
Treatment Outcome
Vaccination
/ methods
Vaccines, Virus-Like Particle
/ administration & dosage
Vero Cells
B cells
COVID-19
S glycoprotein
SARS-CoV-2
antibodies
formaldehyde cross-linking
immunity
macaques
nanoparticles
protection
Journal
Cell reports. Medicine
ISSN: 2666-3791
Titre abrégé: Cell Rep Med
Pays: United States
ID NLM: 101766894
Informations de publication
Date de publication:
15 02 2022
15 02 2022
Historique:
received:
27
07
2021
revised:
26
11
2021
accepted:
19
01
2022
entrez:
2
3
2022
pubmed:
3
3
2022
medline:
12
3
2022
Statut:
epublish
Résumé
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has caused an ongoing global health crisis. Here, we present as a vaccine candidate synthetic SARS-CoV-2 spike (S) glycoprotein-coated lipid vesicles that resemble virus-like particles. Soluble S glycoprotein trimer stabilization by formaldehyde cross-linking introduces two major inter-protomer cross-links that keep all receptor-binding domains in the "down" conformation. Immunization of cynomolgus macaques with S coated onto lipid vesicles (S-LVs) induces high antibody titers with potent neutralizing activity against the vaccine strain, Alpha, Beta, and Gamma variants as well as T helper (Th)1 CD4
Identifiants
pubmed: 35233549
doi: 10.1016/j.xcrm.2022.100528
pii: S2666-3791(22)00028-3
pmc: PMC8784613
doi:
Substances chimiques
Antibodies, Neutralizing
0
Antibodies, Viral
0
COVID-19 Vaccines
0
Immunoglobulin A
0
Immunoglobulin G
0
Liposomes
0
Spike Glycoprotein, Coronavirus
0
Vaccines, Virus-Like Particle
0
spike protein, SARS-CoV-2
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
100528Informations de copyright
© 2022 The Authors.
Déclaration de conflit d'intérêts
The authors declare no competing interests.
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