Starch biosynthesis in guard cells has features of both autotrophic and heterotrophic tissues.
Journal
Plant physiology
ISSN: 1532-2548
Titre abrégé: Plant Physiol
Pays: United States
ID NLM: 0401224
Informations de publication
Date de publication:
01 06 2022
01 06 2022
Historique:
received:
15
12
2021
accepted:
31
01
2022
pubmed:
4
3
2022
medline:
3
6
2022
entrez:
3
3
2022
Statut:
ppublish
Résumé
The pathway of starch synthesis in guard cells (GCs), despite the crucial role starch plays in stomatal movements, is not well understood. Here, we characterized starch dynamics in GCs of Arabidopsis (Arabidopsis thaliana) mutants lacking enzymes of the phosphoglucose isomerase-phosphoglucose mutase-ADP-glucose pyrophosphorylase starch synthesis pathway in leaf mesophyll chloroplasts or sugar transporters at the plastid membrane, such as glucose-6-phosphate/phosphate translocators, which are active in heterotrophic tissues. We demonstrate that GCs have metabolic features of both photoautotrophic and heterotrophic cells. GCs make starch using different carbon precursors depending on the time of day, which can originate both from GC photosynthesis and/or sugars imported from the leaf mesophyll. Furthermore, we unravel the major enzymes involved in GC starch synthesis and demonstrate that they act in a temporal manner according to the fluctuations of stomatal aperture, which is unique for GCs. Our work substantially enhances our knowledge on GC starch metabolism and uncovers targets for manipulating GC starch dynamics to improve stomatal behavior, directly affecting plant productivity.
Identifiants
pubmed: 35238373
pii: 6541642
doi: 10.1093/plphys/kiac087
pmc: PMC9157084
doi:
Substances chimiques
Arabidopsis Proteins
0
Starch
9005-25-8
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
541-556Informations de copyright
© The Author(s) 2022. Published by Oxford University Press on behalf of American Society of Plant Biologists.
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