Photobiomodulation reduces hippocampal apoptotic cell death and produces a Raman spectroscopic "signature".
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2022
2022
Historique:
received:
19
01
2022
accepted:
11
02
2022
entrez:
3
3
2022
pubmed:
4
3
2022
medline:
26
4
2022
Statut:
epublish
Résumé
Apoptotic cell death within the brain represents a significant contributing factor to impaired post-traumatic tissue function and poor clinical outcome after traumatic brain injury. After irradiation with light in the wavelength range of 600-1200 nm (photobiomodulation), previous investigations have reported a reduction in apoptosis in various tissues. This study investigates the effect of 660 nm photobiomodulation on organotypic slice cultured hippocampal tissue of rats, examining the effect on apoptotic cell loss. Tissue optical Raman spectroscopic changes were evaluated. A significantly higher proportion of apoptotic cells 62.8±12.2% vs 48.6±13.7% (P<0.0001) per region were observed in the control group compared with the photobiomodulation group. After photobiomodulation, Raman spectroscopic observations demonstrated 1440/1660 cm-1 spectral shift. Photobiomodulation has the potential for therapeutic utility, reducing cell loss to apoptosis in injured neurological tissue, as demonstrated in this in vitro model. A clear Raman spectroscopic signal was observed after apparent optimal irradiation, potentially integrable into therapeutic light delivery apparatus for real-time dose metering.
Identifiants
pubmed: 35239693
doi: 10.1371/journal.pone.0264533
pii: PONE-D-22-01807
pmc: PMC8893683
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0264533Déclaration de conflit d'intérêts
The development of this novel concept has resulted in a patent pending application from our group relating to the invasive delivery of PBM, together with the use of temporarily implanted apparatus to establish an optimal dose feedback loop via an optical spectroscopic brain interface (UK Patent Application No 2006201.4). There are no other competing interests to declare, including those relating to employment, consultancy, other patents or products in development. The authors confirm that this does not alter our adherence to all PLOS ONE policies on sharing data and materials.
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