Reliability of point-of-care circulating cathodic antigen assay for diagnosing schistosomiasis mansoni in urine samples from an endemic area of Brazil after one year of storage at -20 degrees Celsius.
Journal
Revista da Sociedade Brasileira de Medicina Tropical
ISSN: 1678-9849
Titre abrégé: Rev Soc Bras Med Trop
Pays: Brazil
ID NLM: 7507456
Informations de publication
Date de publication:
2022
2022
Historique:
received:
06
07
2021
accepted:
24
11
2021
entrez:
3
3
2022
pubmed:
4
3
2022
medline:
8
3
2022
Statut:
epublish
Résumé
The World Health Organization recommends reliable point-of-care (POC) diagnostic testing to eliminate schistosomiasis. Lateral flow immunoassay that detects schistosome circulating cathodic antigen (CCA) in urine to establish prevalence thresholds for intervention in endemic areas is recommended. Stored urine may be useful if surveying at-risk populations is delayed or interrupted by unforeseen circumstances, such as the current COVID-19 pandemic. This study evaluated the manufacturer's claim that Schistosoma mansoni infection can be reliably diagnosed in urine samples stored at -20°C for one year. Two-hundred-forty-two subjects from an endemic site in Brazil provided one urine sample each for testing with URINE CCA (SCHISTO) ECO TESTE® (POC-ECO) and one stool sample each for testing with Kato-Katz (KK) and Helmintex® (HTX) as a robust reference standard for infection status. At least 2 ml of urine from each participant was stored at -20°C; after one year, 76 samples were randomly selected for POC-ECO retesting. The POC-ECO agreement between freshly collected and stored urine was inadequate considering trace results as positive (Cohen's kappa coefficient κ = 0.08) and negative (κ = 0.36). POC-ECO accuracy was not significantly greater than that of routine KK (54%; 95% confidence interval: 42.1%-65.5%). The precision and accuracy of POC-ECO have to be optimized in both freshly collected and stored urine before it can be recommended for use in control programs in Brazil.
Sections du résumé
BACKGROUND
BACKGROUND
The World Health Organization recommends reliable point-of-care (POC) diagnostic testing to eliminate schistosomiasis. Lateral flow immunoassay that detects schistosome circulating cathodic antigen (CCA) in urine to establish prevalence thresholds for intervention in endemic areas is recommended. Stored urine may be useful if surveying at-risk populations is delayed or interrupted by unforeseen circumstances, such as the current COVID-19 pandemic. This study evaluated the manufacturer's claim that Schistosoma mansoni infection can be reliably diagnosed in urine samples stored at -20°C for one year.
METHODS
METHODS
Two-hundred-forty-two subjects from an endemic site in Brazil provided one urine sample each for testing with URINE CCA (SCHISTO) ECO TESTE® (POC-ECO) and one stool sample each for testing with Kato-Katz (KK) and Helmintex® (HTX) as a robust reference standard for infection status. At least 2 ml of urine from each participant was stored at -20°C; after one year, 76 samples were randomly selected for POC-ECO retesting.
RESULTS
RESULTS
The POC-ECO agreement between freshly collected and stored urine was inadequate considering trace results as positive (Cohen's kappa coefficient κ = 0.08) and negative (κ = 0.36). POC-ECO accuracy was not significantly greater than that of routine KK (54%; 95% confidence interval: 42.1%-65.5%).
CONCLUSIONS
CONCLUSIONS
The precision and accuracy of POC-ECO have to be optimized in both freshly collected and stored urine before it can be recommended for use in control programs in Brazil.
Identifiants
pubmed: 35239906
pii: S0037-86822022000100303
doi: 10.1590/0037-8682-0389-2021
pmc: PMC8909435
pii:
doi:
Substances chimiques
Antigens, Helminth
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0389Références
Rev Soc Bras Med Trop. 2020 Oct 05;53:e20200411
pubmed: 33027417
Biochem Med (Zagreb). 2012;22(3):276-82
pubmed: 23092060
Parasitology. 2020 Sep;147(10):1140-1148
pubmed: 32484122
Acta Trop. 2016 Dec;164:208-215
pubmed: 27647573
Mem Inst Oswaldo Cruz. 2019;114:e180529
pubmed: 31166420
PLoS Negl Trop Dis. 2018 Feb 22;12(2):e0006232
pubmed: 29470516
Am J Trop Med Hyg. 2020 Jul;103(1):315-324
pubmed: 32431276
J Clin Diagn Res. 2016 Oct;10(10):YE01-YE06
pubmed: 27891446
Parasite Epidemiol Control. 2021 Mar 02;13:e00208
pubmed: 33732914
Parasitol Int. 2021 Feb;80:102201
pubmed: 33010472
Parasitology. 2021 Apr;148(4):420-426
pubmed: 33190646
Acta Trop. 2019 Sep;197:105049
pubmed: 31158344
Am J Trop Med Hyg. 2019 Mar;100(3):578-583
pubmed: 30608053
Rev Soc Bras Med Trop. 2020 Sep 25;53:e20190562
pubmed: 32997046
Exp Parasitol. 2017 Jun;177:28-34
pubmed: 28431921
Trans R Soc Trop Med Hyg. 2018 Jan 1;112(1):1-7
pubmed: 29522211
Acta Trop. 2021 May;217:105863
pubmed: 33587944
BMC Public Health. 2018 Apr 11;18(1):478
pubmed: 29642875
Rev Inst Med Trop Sao Paulo. 1972 Nov-Dec;14(6):397-400
pubmed: 4675644
PLoS Negl Trop Dis. 2020 Aug 25;14(8):e0008473
pubmed: 32841228
Acta Trop. 2018 Jun;182:264-270
pubmed: 29526480
J Thorac Oncol. 2010 Jun;5(6):763-4
pubmed: 20502268
PLoS Negl Trop Dis. 2018 Nov 8;12(11):e0006813
pubmed: 30408030
J Immunol Methods. 2019 Sep;472:1-6
pubmed: 31194970
Anal Biochem. 2002 May 15;304(2):206-11
pubmed: 12009697
Fam Med. 2005 May;37(5):360-3
pubmed: 15883903
Clin Infect Dis. 2021 Apr 26;72(8):1463-1466
pubmed: 32984870
PLoS Negl Trop Dis. 2021 Jul 14;15(7):e0009569
pubmed: 34260610
Rev Soc Bras Med Trop. 2016 May-Jun;49(3):341-7
pubmed: 27384831
Acta Trop. 2019 Nov;199:105150
pubmed: 31425672
Biometrics. 1977 Mar;33(1):159-74
pubmed: 843571
Am J Trop Med Hyg. 2020 Jul;103(1_Suppl):42-49
pubmed: 32400347
BMC Infect Dis. 2020 Apr 22;20(1):301
pubmed: 32321418
PLoS Negl Trop Dis. 2018 Mar 8;12(3):e0006274
pubmed: 29518081