Epithelial RAC1-dependent cytoskeleton dynamics controls cell mechanics, cell shedding and barrier integrity in intestinal inflammation.
IBD
actin cytoskeleton
epithelial barrier
intestinal epithelium
tight junction
Journal
Gut
ISSN: 1468-3288
Titre abrégé: Gut
Pays: England
ID NLM: 2985108R
Informations de publication
Date de publication:
02 2023
02 2023
Historique:
received:
30
06
2021
accepted:
29
01
2022
pubmed:
5
3
2022
medline:
10
1
2023
entrez:
4
3
2022
Statut:
ppublish
Résumé
Increased apoptotic shedding has been linked to intestinal barrier dysfunction and development of inflammatory bowel diseases (IBD). In contrast, physiological cell shedding allows the renewal of the epithelial monolayer without compromising the barrier function. Here, we investigated the role of live cell extrusion in epithelial barrier alterations in IBD. Taking advantage of conditional GGTase and RAC1 knockout mice in intestinal epithelial cells ( Epithelial Impaired epithelial RAC1 function causes cell overcrowding and epithelial leakage thus inducing chronic intestinal inflammation. Epithelial RAC1 emerges as key regulator of cytoskeletal dynamics, cell mechanics and intestinal cell shedding. Modulation of RAC1 might be exploited for restoration of epithelial integrity in the gut of patients with IBD.
Identifiants
pubmed: 35241625
pii: gutjnl-2021-325520
doi: 10.1136/gutjnl-2021-325520
pmc: PMC9872254
doi:
Substances chimiques
rac1 GTP-Binding Protein
EC 3.6.5.2
RAC1 protein, human
0
Rac1 protein, mouse
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
275-294Commentaires et corrections
Type : CommentIn
Informations de copyright
© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.
Déclaration de conflit d'intérêts
Competing interests: None declared.
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