Sex-linked deubiquitinase establishes uniparental transmission of chloroplast DNA.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
03 03 2022
03 03 2022
Historique:
received:
27
08
2021
accepted:
09
02
2022
entrez:
4
3
2022
pubmed:
5
3
2022
medline:
13
4
2022
Statut:
epublish
Résumé
Most sexual organisms inherit organelles from one parent, commonly by excluding organelles from the smaller gametes. However, post-mating elimination of organelles derived from one gamete ensures uniparental inheritance, where the underlying mechanisms to distinguish organelles by their origin remain obscure. Mating in Chlamydomonas reinhardtii combines isomorphic plus and minus gametes, but chloroplast DNA from minus gametes is selectively degraded in zygotes. Here, we identify OTU2p (otubain protein 2), encoded in the plus mating-type locus MT+, as the protector of plus chloroplast. Otu2p is an otubain-like deubiquitinase, which prevents proteasome-mediated degradation of the preprotein translocase of the outer chloroplast membrane (TOC) during gametogenesis. Using OTU2p-knockouts and proteasome inhibitor treatment, we successfully redirect selective DNA degradation in chloroplasts with reduced TOC levels regardless of mating type, demonstrating that plus-specific Otu2p establishes uniparental chloroplast DNA inheritance. Our work documents that a sex-linked organelle quality control mechanism drives the uniparental organelle inheritance without dimorphic gametes.
Identifiants
pubmed: 35241655
doi: 10.1038/s41467-022-28807-6
pii: 10.1038/s41467-022-28807-6
pmc: PMC8894339
doi:
Substances chimiques
DNA, Chloroplast
0
Deubiquitinating Enzymes
EC 3.4.19.12
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
1133Informations de copyright
© 2022. The Author(s).
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