Abdominal multiorgan procurement from slaughterhouse pigs: a bespoke model in organ donation after circulatory death for
Transplantation
donation after circulatory death (DCD)
machine perfusion
organ preservation
tissue and organ harvesting
Journal
Annals of translational medicine
ISSN: 2305-5839
Titre abrégé: Ann Transl Med
Pays: China
ID NLM: 101617978
Informations de publication
Date de publication:
Jan 2022
Jan 2022
Historique:
received:
15
05
2021
accepted:
19
07
2021
entrez:
4
3
2022
pubmed:
5
3
2022
medline:
5
3
2022
Statut:
ppublish
Résumé
Advances in organ preservation, reconditioning and assessment have been driven by the increasing necessity to utilise organs from extended criteria donors, particularly donors after circulatory death. Research efforts in this area have aided translation of machine perfusion technology into clinical practice. Pigs are anatomically and physiologically similar to humans and are an excellent model. However, conducting large animal experimental research is challenging and typically limited by ethical and economic constraints. Here we describe a reproducible, cost-effective multi-organ abdominal procurement model of porcine organs from the slaughterhouse. Domestic pigs are electrically stunned and exsanguinated following the standard abattoir process. Via a longitudinal midline incision, the thoracoabdominal viscera are removed The warm ischaemic time is kept between 15-30 minutes. Using this highly protocolized procurement technique we have procured 12 livers, 162 kidneys and 12 pancreata for research, the majority of which have been utilized for We have described a reliable and reproducible procedure for abdominal multi-organ procurement from slaughterhouse pigs.
Sections du résumé
BACKGROUND
BACKGROUND
Advances in organ preservation, reconditioning and assessment have been driven by the increasing necessity to utilise organs from extended criteria donors, particularly donors after circulatory death. Research efforts in this area have aided translation of machine perfusion technology into clinical practice. Pigs are anatomically and physiologically similar to humans and are an excellent model. However, conducting large animal experimental research is challenging and typically limited by ethical and economic constraints. Here we describe a reproducible, cost-effective multi-organ abdominal procurement model of porcine organs from the slaughterhouse.
METHODS
METHODS
Domestic pigs are electrically stunned and exsanguinated following the standard abattoir process. Via a longitudinal midline incision, the thoracoabdominal viscera are removed
RESULTS
RESULTS
The warm ischaemic time is kept between 15-30 minutes. Using this highly protocolized procurement technique we have procured 12 livers, 162 kidneys and 12 pancreata for research, the majority of which have been utilized for
CONCLUSIONS
CONCLUSIONS
We have described a reliable and reproducible procedure for abdominal multi-organ procurement from slaughterhouse pigs.
Identifiants
pubmed: 35242846
doi: 10.21037/atm-21-2494
pii: atm-10-01-1
pmc: PMC8825551
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1Informations de copyright
2022 Annals of Translational Medicine. All rights reserved.
Déclaration de conflit d'intérêts
Conflicts of Interest: All authors have completed the ICMJE uniform disclosure form (available at https://dx.doi.org/10.21037/atm-21-2494). The authors have no conflicts of interest to declare.
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