scGR-seq: Integrated analysis of glycan and RNA in single cells.
Cell Biology
Cell culture
Genomics
RNAseq
Sequencing
Single Cell
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
18 03 2022
18 03 2022
Historique:
entrez:
4
3
2022
pubmed:
5
3
2022
medline:
9
4
2022
Statut:
epublish
Résumé
Glycans are structurally diverse molecules found on the surface of living cells. The protocol details a system developed for combined analysis of glycan and RNA in single cells (scGR-seq) using human induced pluripotent stem cells (hiPSCs) and hiPSC-derived neural progenitor cells (NPCs). scGR-seq consists of DNA-barcoded lectin-based glycan profiling by sequencing (scGlycan-seq) and single-cell transcriptome profiling (scRNA-seq). scGR-seq will be an essential technique to delineate the cellular heterogeneity of glycans across multicellular systems. For complete details on the use and execution of this profile, please refer to Minoshima et al. (2021).
Identifiants
pubmed: 35243371
doi: 10.1016/j.xpro.2022.101179
pii: S2666-1667(22)00059-4
pmc: PMC8861808
doi:
Substances chimiques
Polysaccharides
0
RNA
63231-63-0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101179Informations de copyright
© 2022 The Author(s).
Déclaration de conflit d'intérêts
The authors declare no competing interests.
Références
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pubmed: 34401666
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pubmed: 29126257
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pubmed: 21471226