Generation of a GPR146 knockout human induced pluripotent stem cell line (ITXi001-A-1).
Journal
Stem cell research
ISSN: 1876-7753
Titre abrégé: Stem Cell Res
Pays: England
ID NLM: 101316957
Informations de publication
Date de publication:
04 2022
04 2022
Historique:
received:
13
12
2021
accepted:
23
02
2022
pubmed:
6
3
2022
medline:
16
4
2022
entrez:
5
3
2022
Statut:
ppublish
Résumé
Dyslipidemia is a key modifiable causal risk factor involved in the development of atherosclerotic cardiovascular disease. Recently, the G protein-coupled receptor 146 (GPR146), a member of the G-coupled protein receptors' family, has been shown to be a regulator of plasma cholesterol. Inhibition of hepatic GPR146 in mice displays protective effect against both hypercholesterolemia and atherosclerosis. Here, we characterize a genetically engineered human induced pluripotent stem cell (hiPSC) model invalidated for GPR146 (ITXi001-A-1) using CRISPR-Cas9 editing technology. Differentiation of ITXi001-A-1 towards hepatic fate will provide a suitable model for deciphering the molecular mechanisms sustaining the beneficial metabolic effects of GPR146 inhibition.
Identifiants
pubmed: 35247835
pii: S1873-5061(22)00070-8
doi: 10.1016/j.scr.2022.102721
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
102721Informations de copyright
Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.