CPHEN-013: Comprehensive phenotyping of hematopoietic stem and progenitor cells in the human fetal liver.
flow cytometry
hematopoiesis
hematopoietic stem cells
immunophenotyping panel
spectral cytometry
Journal
Cytometry. Part A : the journal of the International Society for Analytical Cytology
ISSN: 1552-4930
Titre abrégé: Cytometry A
Pays: United States
ID NLM: 101235694
Informations de publication
Date de publication:
11 2022
11 2022
Historique:
revised:
21
12
2021
received:
01
10
2021
accepted:
11
01
2022
pubmed:
8
3
2022
medline:
3
11
2022
entrez:
7
3
2022
Statut:
ppublish
Résumé
Hematopoietic stem cells (HSCs) reside at the top of the hematopoietic hierarchy and can give rise to all the mature blood cell types in our body, while at the same time maintaining a pool of HSCs through self-renewing divisions. This potential is reflected in their functional definition as cells that are capable of long-term multi-lineage engraftment upon transplantation. While all HSCs meet these criteria, subtle differences exist between developmentally different populations of these cells. Here we present a comprehensive overview of traditional and more recently described markers for phenotyping HSCs and their downstream progeny. To address the need to assess the growing number of surface molecules expressed in various HSC-enriched fractions at different developmental stages, we have developed an extensive multi-parameter spectral flow cytometry panel to phenotype hematopoietic stem and multipotent progenitor cells (HSC/MPPs) throughout development. In this study we then employ this panel to comprehensively profile the HSC compartment in the human fetal liver (FL), which is endowed with superior engraftment potential compared to postnatal sources. Spectral cytometry lends an improved resolution of marker expression to our comprehensive approach, allowing to extract combinatorial expression signatures of several relevant HSC/MPP markers to precisely characterize the HSC/MPP fraction in a variety of tissues.
Identifiants
pubmed: 35253987
doi: 10.1002/cyto.a.24540
doi:
Substances chimiques
Biomarkers
0
Types de publication
Review
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
903-908Informations de copyright
© 2022 International Society for Advancement of Cytometry.
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