Meta-signature guided investigation of miRNA candidates as potential biomarkers of oral cancer.
miR-135b-5p
miR-139-5p
miR-18a-5p
miR-30a-5p
miR-31-3p
oral cancer
Journal
Oral diseases
ISSN: 1601-0825
Titre abrégé: Oral Dis
Pays: Denmark
ID NLM: 9508565
Informations de publication
Date de publication:
May 2023
May 2023
Historique:
revised:
25
02
2022
received:
29
09
2021
accepted:
04
03
2022
medline:
21
4
2023
pubmed:
10
3
2022
entrez:
9
3
2022
Statut:
ppublish
Résumé
This study aimed to experimentally validate dysregulated expression of miRNA candidates selected through updated meta-analysis of most commonly deregulated miRNAs in oral cancer and to explore their diagnostic and prognostic potential. Five miRNAs (miR-31-3p, miR-135b-5p, miR-18a-5p, miR-30a-5p and miR-139-5p) from updated meta-signature were selected for validation by qRT-PCR method in 35 oral cancer clinical specimens and adjacent non-cancerous tissue. Updated meta-analysis has identified 13 most commonly deregulated miRNAs in oral cancer. Seven miRNAs were consistently up-regulated (miR-21-5p, miR-31-3p, miR-135b-5p, miR-31-5p, miR-424-5p, miR-18a-5p and miR-21-3p), while five were down-regulated (miR-139-5p, miR-30a-3p, miR-375-3p, miR-376c-3p and miR-30a-5p). Increased expression of miR-31-3p and miR-135b-5p, and decreased expression of miR-139-5p and miR-30a-5p were confirmed in oral cancer compared to adjacent non-cancerous tissue. A three miRNAs combination (miR-31-3p, miR-139-5p and miR-30a-5p) gave the most promising diagnostic potential for discriminating oral cancer from non-cancerous tissue (AUC: 0.780 [95% CI: 0.673-0.886], p < 0.0005, sensitivity 94.3%, specificity 51.4%). High expression of miR-135b-5p, miR-18a-5p and miR-30a-5p was associated with poor survival (p = 0.003, p = 0.048, p = 0.016 respectively). miR-31-3p, miR-139-5p and miR-30a-5p panel was confirmed as a potential diagnostic biomarker when distinguishing oral cancer from non-cancerous tissue. miR-135b-5p, miR-18a-5p and miR-30a-5p might serve as potential biomarkers of poor survival of oral cancer patients.
Substances chimiques
MicroRNAs
0
Biomarkers, Tumor
0
MIRN139 microRNA, human
0
Types de publication
Meta-Analysis
Journal Article
Langues
eng
Pagination
1550-1564Subventions
Organisme : Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja
ID : 451-03-68/2022-14/ 200017
Organisme : Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja
ID : 451-03-68/2022-14/200178
Informations de copyright
© 2022 Wiley Periodicals LLC.
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